首页 > 学术百科

FGF23对破骨细胞分化和功能的影响

摘要 (1)

Abstract (2)

1 前言 (3)

1.1 破骨细胞分化与功能 (3)

1.1.1 破骨细胞来源 (3)

1.1.2 破骨细胞分化过程 (4)

1.1.3 调控破骨细胞分化重要因子 (5)

1.1.4 调控破骨细胞分化信号通路 (6)

1.1.5 破骨细胞功能 (7)

1.2 FGF23-Klotho轴在骨矿物质代谢中的作用 (10)

1.2.1 FGF23-Klotho轴的生物学研究 (10)

1.2.2 FGF23-Klotho轴参与骨矿物质代谢调节 (11)

1.2.3 FGF23-Klotho轴对骨代谢的重要意义 (13)

1.3 本研究的目的与意义 (14)

2 材料与方法 (15)

2.1 试验材料 (15)

2.1.1 试验细胞株 (15)

2.1.2 主要试剂 (15)

2.1.3 试验主要仪器及设备 (16)

2.2 试验方法 (16)

2.2.1 主要溶液配置 (16)

2.2.2 RAW264.7细胞培养、诱导分化及鉴定 (16)

2.2.3 试验分组与处理 (17)

2.2.4 破骨细胞TRAP染及计数 (18)

2.2.5 破骨细胞TRAP活性检测 (18)

2.2.6 破骨细胞骨吸收活性检测 (19)

2.2.7 RT-PCR检测破骨细胞功能相关基因mRNA表达水平 (19)

2.2.8 统计学分析 (21)

3 结果与分析 (22)

3.1 破骨细胞分化鉴定 (22)

3.2 FGF23对不同时期破骨细胞数量的影响 (22)

3.2.1 第3天TRAP染结果 (22)

3.2.2 第5天TRAP染结果 (24)

3.2.3 第7天TRAP染结果 (25)

3.2.4 第9天TRAP染结果 (26)

3.3 FGF23对不同时期破骨细胞TRAP活性的影响 (27)

3.4 FGF23对骨吸收活性的影响 (28)

3.5 FGF23对不同时期破骨细胞主要功能相关基因mRNA表达的影响 (29)

3.5.1 破骨细胞Fgfrs和Klotho相关基因表达 (29)

3.5.2 破骨细胞骨吸收标志物相关基因表达 (32)

3.5.3 破骨细胞分泌调控成骨细胞的活动因子相关基因表达 (35)

4 讨论 (39)

4.1 破骨细胞体外培养方法的确定 (39)

4.1.1 破骨细胞体外培养体系的建立 (39)

4.1.2 破骨细胞前体培养方式的选择 (40)

4.2 FGF23对OC分化的影响 (41)

4.3 FGF23对OC表达的Fgfrs和Klotho的影响 (43)

4.4 FGF23对OC介导的骨吸收影响 (44)

4.5 FGF23对OC分泌的调控OB功能相关因子表达的影响 (46)

5 结论 (49)

参考文献 (50)

致谢 (57)

攻读学位期间发表的文章 (58)

CONTENTS

Chinese Abstract (1)

Abstract (2)

1 Introduction (3)

1.1 The differentiation and function of osteoclasts (3)

1.1.1 The source of osteoclasts (3)

1.1.2 The process of osteoclast differentiation (4)

1.1.3 Regulation of the important factor of osteoclast differentiation (5)

spe1.1.4 Regulation of the signal pathway of osteoclast differentiation (6)

1.1.5 The function of osteoclasts (7)

1.2 The role of FGF23-Klotho axis in bone mineral metabolism (10)

1.2.1 Biological research of FGF23-Klotho axis (10)

1.2.2 The FGF23-Klotho axis is involved in bone mineral metabolism (11)

1.2.3 The significance of FGF23-Klotho axis to bone metabolism (13)

1.3 The purpose and significance of the study (14)

2 Materials and Methods (15)

2.1 Experimental materials (15)

2.1.1 Experimental cell line (15)

2.1.2 Major reagents (15)

2.1.3 Instruments and equipments (16)

2.2 Experimental methods (16)

2.2.1 The main solution configuration (16)

2.2.2 Culture RAW264.7 cells of differentiation and identification (16)

2.2.3Experimental designs and treatments (17)

2.2.4TRAP staining and counting of osteoclast (18)

2.2.5 Detection of TRAP activity in osteoclasts (18)

2.2.6 Detection of bone resorption activity in osteoclast (19)

2.2.7 Detection of osteoclast function related gene mRNA expression by RT-PCR 19

2.2.8 Statistical analysis (21)

3 Results and Analysis (22)

3.1 Identification of osteoclast differentiation (22)

3.2 The effect of FGF23 on the number of osteoclasts at different times (22)

3.2.1 TRAP staining results at the third day (22)

3.2.2 TRAP staining results at the fifth day (24)

3.2.3 TRAP staining results at the seventh day (25)

3.2.4 TRAP staining results at the ninth day (26)

3.3 Effects of FGF23 on osteoclast of TRAP activity at different times (27)

3.4 Effects of FGF23 on osteoclast of bone resorption activity (28)电脑故障诊断大师

III

3.5 Effects of FGF23 on osteoclasts at different stages the major function gene mRNA

expression (29)

3.5.1 The expression of Fgfrs and Klotho in osteoclast (29)

3.5.2 The expression of differentiation maturing markers in osteoclast (32)

3.5.3 The expression of regulating osteoblast secreted by osteoclast (35)鹿心社简历

4 Discussion (39)

4.1 Determination of osteoclast culture in vitro (39)

4.1.1 Establishment of osteoclast culture system in vitro (39)

4.1.2 Selection of the culture methods of osteoclast precursor (40)

4.2 The effect of FGF23 on osteoclast differentiation (41)

4.3 The effect of FGF23 on the Fgfrs and Klotho expression (43)

4.4 The effect of FGF23 on bone resorption mediated by osteoclast (44)

fgf4.5 The effect of FGF23 on the regulation of the function of osteoblast secreted by

osteoclast (46)

5 Conclusion (49)

Reference (50)

Acknowledgments (57)

Published Essay (58)

英文缩略表

英文缩写英文全称中文名称FGF23 Fibroblast growth factor 23 成纤维细胞生长因子23

OC osteoclast cell 破骨细胞

OB osteoblast cell 成骨细胞

RANKL Receptor Activator for Nuclear Factor-κB Ligand核因子κB受体活化因子配体M-CSF Macrophage colony stimulating factor 巨噬细胞集落刺激因子蔡星辰

FGFRs Fibroblast growth factor receptors 成纤维细胞生长因子受体ATP6I ATPase subunit 6I 氢离子三磷酸腺苷转运酶CtsK Cathepsin K 组织蛋白酶K

TRAP Tartrate resistant acid phosphatase 抗酒石酸酸性磷酸酶

MMP-9 Matrix metalloproteinase-9 基质金属蛋白酶

BMPs Bonemorphogenetic proteins 骨形态发生蛋白

SPHK1 Sphingosine kinase 1 神经鞘氨醇激酶

司法鉴定机构登记管理办法

PDGF-bb Platelet-derived growth factor-bb 血小板衍生生长因子

本文发布于:2024-09-20 20:25:13，感谢您对本站的认可！

本文链接：https://www.17tex.com/xueshu/723787.html

上一篇：新型糖尿病药物FGF-21

下一篇：一种FGF21蛋白类似物及其应用[发明专利]

标签：骨细胞相关试验分化表达因子基因功能

留言与评论（共有 0 条评论）