罗丹明显培养基：
甲组分：0.1% (NH4)2SO4，0.1% K2HPO4如何当好一把手
，0.5% KCl，0.05% MgSO4·7H2O，0.01% FeSO4·7H2O，0.5% YE，0.5% Trypton， 2% 琼脂， pH血氧探头
精加力自然，装入三角瓶中，115℃灭菌30 min； 乙组分：取橄榄油15ml与2％的聚乙烯醇（PVA）溶液50ml混合1:3的比例混合，用细胞破碎仪10000rpm破碎乳化3min，间歇5min，再次乳化3min，115℃灭菌30 min；
将甲、乙组分溶液灭菌后，冷却至60℃ 将12ml乙组份加入到100ml甲组份混匀。 Rhodamine B：使用时加入0.寻成龙首映
1g罗丹明溶解在100mL用灭菌的针头滤器(0.2μm)过滤除菌，0.1%浓度的Rhodamine B溶液以1：100体积比加入到甲乙混合液中混匀，作为显指示剂倒平板。 Rhodamine B Plate screening Culture Medium：
Component A: 0.1% (NH4)2SO4, 0.1% K2HPO4, 0.5% KCl, 0.05% MgSO4·7H2O, 0.01% F
eSO4·7H2O, 0.5% YE, 0.5% Trypton, 2% agar resoluted in beaker without changing the pH, then the solution will be loaded into baffled flask followed by sterilizing at 115℃ for 30 min;
Component B: dissolve 2% polyvinyl alcohol（PVA私语者） into water by heating to boiling for about 30 min until totally dissolved, put 15 mL palmitic oil and 50 mL 2% PVA solution together, emulsify the oils by 10000 r/min for 15 min using emulsifying equipment, then sterilize the compound for 30 min;
While the component A and B are cold to about 60℃ after the sterilizing, mix 12 mL component A with 100 mL component B;
Rhodamine B: dissolve 0.1 g Rhodamine B to 100 mL sterilized water, sterilizing the resolution by filtration using sterilized syringe whose diameter of sieve pore is 0.2μm, then the 0.1% Rhodamine B solution, as the coloring indicator of the plate screening culure, will be mixed with the compound of component A and B with a rate of 1甾醇：100 in volume, then pour the compound with Rhodamine B into the sterilized plates.
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