高效液相谱法测定甜菜碱的含量昂达v711作者：张倩茹 尹蓉 殷龙龙 王贤萍 孙慧英 梁志宏来源：《安徽农学通报》2019年第15期 摘 要：选用Supersil SCX 5μm高效液相谱柱，柱温30℃，流动相为0.01M甲酸铵-
甲醇∶水85∶15（v/v）溶液，流速1.0mL/min，建立了高效液相谱-示差检测器测定甜菜碱的方法。该方法线性关系良好，其线性回归方程为y=108284x-23952，相关系数R2=0.9994，甜菜碱的加标回收率为99.45%～100.63%，为甜菜碱的检测提供了一种快速高效的方法，且灵敏度高，重复性好，简便易行。 刘彭芝 关键词：高效液相谱;甜菜碱;甲醇
mediaringtalk 中图分类号 S566.3 文献标识码 A 文章编号 1007-7731（2019求学商城）15-0011-03西部开发的资料
Determination of Betaine by High Performance Liquid Chromatography
Zhang Qianru1 et al.
（1Institute of Pomology，Shanxi Academy of Agricultural Sciences，Shanxi Key Laboratory of Germplasm Improvementand Utilization in Pomology，系统工程与电子技术Taiyuan 030031，China）
Abstract：An high performance liquid chromatography method for determination of b
etaine was built up. The Supersil SCX 5μm high performance liquid chromatography column was selected，the column was set at 40℃，using 0.01mol/L ammonium formate methanol∶water 85∶15（v/v） solution as mobile phase at a flow rate of 1.0mL/min. The results showed that the method has a good linear relationship and high sensitivity，y=108284x-23952，R2=0.9994，the recovery rate between 99.45%-100.63%.This method provided a fast and efficient for determination of betaine，which was sensitive repeatable and easy to use.
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