NEB公司提供的保护碱基序列表

NEB公司提供保护碱基序列表:
铁锰结核
Enzyme
Oligo Sequence
Chain 
Length
% Cleavage
2 hr
20 hr
AccI
GGTCGAC
CGGTCGACCG 
CCGGTCGACCGG
10 
12
0
0
AflIII
CACATGT
CCACATGTGG 
CCCACATGTGGG
10 
12
>90 
>90
>90 
>90
AscI
GGCGCGCC 
AGGCGCGCC
TTGGCGCGCCAA
10 
12
>90 
>90 
>90
>90 
>90 
>90
AvaI
CCCCGGG
CCCCCGGGGG 
TCCCCCGGGGGA
10 
12
50 
>90 
>90
>90 
>90 
>90
BamHI
CGGATCC
CGGGATCCCG 
CGCGGATCCGCG
10 
12
10 
>90 
>90
25 
>90 
>90
BglII
CAGATCT
GAAGATCTTC 
GGAAGATCTTCC
船舶
10 
12
75 
25
>90 
>90
BssHII
GGCGCGC
AGGCGCGCCT 
TTGGCGCGCCAA
10 
12
50
>90
BstEII
GGGT(A/T)ACCC
9
0
10
BstXI
AACTGCAGAACCAATGCATTGG
AAAACTGCAGCCAATGCATTGGAA 
CTGCAGAACCAATGCATTGGATGCAT
22 
24 
27
25 
25
50 
>90
ClaI
CATCGAT
GATCGAT
CCATCGATGG 
CCCATCGATGGG
10 
12
>90 
50
>90 
50
Enzyme
Oligo Sequence
Chain 
Length
% Cleavage
2 hr
20 hr
EcoRI
GGAATTC
CGGAATTCCG 
CCGGAATTCCGG
10 
12
>90 
>90 
>90
>90 
>90 
>90
HaeIII
GGGGCCCC 
AGCGGCCGCT 
TTGCGGCCGCAA
10 
12
>90 
>90 
>90
>90 
>90 
>90
HindIII
CAAGCTT
CCAAGCTTGG 
CCCAAGCTTGGG
10 
12
10
75
KpnI
GGGTACC
GGGGTACCCC 
CGGGGTACCCCG
10 
12
>90 
>90
>90 
>90
MluI
GACGCGT
CGACGCGTCG
10
25
50
NcoI
CCCATGG
CATGCCATGGCATG
14
50
75
NdeI
CCATATG
CCCATATGGG 
CGCCATATGGCG 
GGGTTTCATATGAAACCC 
GGAATTCCATATGGAATTCC 
GGGAATTCCATATGGAATTCCC
10 
12 
18 
20 
22
75 
75
>90 
>90
NheI
GGCTAGC
CGGCTAGCCG 
CTAGCTAGCTAG
10 
12
10 
10
25 
50
NotI
TTGCGGCCGCAA 
ATTTGCGGCCGCTTTA 
AAATATGCGGCCGCTATAAA 
ATAAGAATGCGGCCGCTAAACTAT 
AAGGAAAAAAGCGGCCGCAAAAGGAAAA
12 
16 
20 
24 
28
10 
10 
25 
25
10 
10 
90 
>90
Enzyme
Oligo Sequence
Chain 
Length
% Cleavage
2 hr
20 hr
NsiI
TGCATGCATGCA 
CCAATGCATTGGTTCTGCAGTT
12 
22
10 
>90
>90 
>90
PacI
TTAATTAA 
GTTAATTAA
CCTTAATTAAGG
10 
12
0
25 
>90
PmeI
GTTTAAAC 
GGTTTAAAC
GGGTTTAAACCC 
AGCTTTGTTTAAACGGCGCGCCGG
10 
12 
24
75
25 
50 
>90
PstI
GCTGCAG
TGCACTGCAGTGCA 
AACTGCAGAACCAATGCATTGG
AAAACTGCAGCCAATGCATTGGAA 
CTGCAGAACCAATGCATTGGATGCAT
14 
22 
24 
26
10 
>90 
>90 
0
10 
>90 
>90 
0
PvuI
CCGATCG
ATCGATCGAT 
TCGCGATCGCGA
10 
12
10 
0
25 
10
SacI
CGAGCTCG
8
10
10
SacII
GCCGCGG
TCCCCGCGGGGA
12
50
>90
SalI
GTCGACGTCAAAAGGCCATAGCGGCCGC 
GCGTCGACGTCTTGGCCATAGCGGCCGCGG 
ACGCGTCGACGTCGGCCATAGCGGCCGCGGAA
28 
30 
32
10 
10
50 
75
ScaI
GAGTACT
AAAAGTACTTTT
12
10 
75
25 
75
SmaI
CCCGGG 
CCCCGGG
CCCCCGGGGG 
TCCCCCGGGGGA
10 
12
10 
>90
10 
10 
50 
>90
Enzyme
Oligo Sequence
Chain 
Length
% Cleavage
2 hr
20 hr
SpeI
GACTAGT
GGACTAGTCC 
CGGACTAGTCCG 
CTAGACTAGTCTAG
10 
12 
14
10 
10 
0
>90 
>90 
50 
50
SphI
GGCATGC
CATGCATGCATG 
ACATGCATGCATGT
12 
14
10
25 
50
StuI
AAGGCCT
GAAGGCCTTC 
AAAAGGCCTTTT
10 
12
>90 
>90 
>90
>90 
>90 
>90
XbaI
CTCTAGA
GCTCTAGAGC 
TGCTCTAGAGCA 
CTAGTCTAGACTAG
10 
12 
14
>90 
75 
75
>90 
>90 
>90
XhoI
CCTCGAG
CCCTCGAGGG 
CCGCTCGAGCGG
10 
12
10 
10
25 
75
XmaI
CCCCGGG
CCCCCGGGGG 
CCCCCCGGGGGG 
TCCCCCCGGGGGGA
10 
12 
14
25 
50 
>90
75 
>90 
>90
四. Fermentas公司提供的关于保护碱基的资料,其实这个更有参考价值~
太多了,请参考:
www.fermentas/en/support/technical-reference/restriction-enzymes/cleavage-efficiency
NEB公司的请参考:
b/nebecomm/tech_reference/restriction_enzymes/cleavage_linearized_vector.asp
b/nebecomm/tech_reference/restriction_enzymes/cleavage_olignucleotides.asp#A
Table. Cleavage Efficiency Close to the Termini of PCR Fragments
中国经纪人登录
Enzyme
Bp from the recognition site to fragment end
1
2
3
4
5
AanI (PsiI)
0
0-20
20-50
AarI
20-50
50-100
AasI (DrdI)
50-100
AatII
0
0-20
20-50
50-100
Acc65I (Asp718I)
0-20
50-100
AdeI (DraIII)
50-100
AjiI (BmgBI)
50-100
AluI
0-20
20-50
50-100
Alw21I (BsiHKAI)
50-100
Alw26I
50-100
Alw44I
0
20-50
50-100
ApaI
50-100
BamHI
50-100
BauI (BssSI)
0-20
20-50
50-100
BclI
0
50-100
BcnI (NciI)
20-50
lonely boy
50-100
BcuI (SpeI)
50-100
BfiI (BmrI)
50-100
BfmI (SfcI)
50-100
BfuI (BciVI)
50-100
BglI
20-50
50-100
BglII
0
50-100
Bme1390I (ScrFI)
20-50
50-100
BoxI (PshAI)
0
50-100
BpiI (BbsI)
50-100
Bpu10I
20-50
50-100
Bpu1102I (BlpI)
50-100
BseDI (BsaJI)
0
50-100
BseGI (BtsCI)
50-100
BseJI (BsaBI)
0
50-100
BseLI (BslI)
0
50-100
BseMI (BsrDI)
0-20
50-100
BseMII (BspCNI)
50-100
BseNI (BsrI)
0
50-100
BseSI (Bme1580I)
50-100
BseXI (BbvI)
20-50
50-100
Bsh1236I (BstUI)
50-100
Bsh1285I (BsiEI)
havd-686
0-20
50-100
语音芯片
BshNI (BanI)
50-100
BshTI (AgeI)
20-50
50-100
Bsp119I (BstBI)
50-100
Bsp120I (PspOMI)
20-50
50-100
Bsp1407I (BsrGI)
20-50
50-100
Bsp143I (Sau3AI)
50-100
Bsp68I (NruI)
0
50-100
BspLI (NlaIV)
50-100
BspOI (BmtI)
0
20-50
50-100
BspPI (AlwI)
0
50-100
BspTI (AflII)
0
0-20
50-100
Bst1107I (BstZ17I)
0-20
50-100
BstXI
0
50-100
Bsu15I (ClaI)
50-100
BsuRI (HaeIII)
0-20
20-50
50-100
BveI (BspMI)
0-20
50-100
CaiI (AlwNI)
0
0-20
50-100
Cfr10I (BsrFI)
20-50
50-100
Cfr13I (Sau96I)
50-100
Cfr42I (SacII)
50-100

本文发布于:2024-09-21 13:23:42,感谢您对本站的认可!

本文链接:https://www.17tex.com/xueshu/579321.html

版权声明:本站内容均来自互联网,仅供演示用,请勿用于商业和其他非法用途。如果侵犯了您的权益请与我们联系,我们将在24小时内删除。

标签:保护   碱基   提供   公司
留言与评论(共有 0 条评论)
   
验证码:
Copyright ©2019-2024 Comsenz Inc.Powered by © 易纺专利技术学习网 豫ICP备2022007602号 豫公网安备41160202000603 站长QQ:729038198 关于我们 投诉建议