NEB保护碱基-各种酶切位点保护碱基PCR设计引物时酶切位点的保护 酶寡核苷酸序列
切割率%
2 hr20 hr
Acc I G GTCGAC C
CG GTCGAC CG
CCG GTCGAC CGG 0
Afl III C ACATGT G
CC ACATGT GG
CCC ACATGT GGG
>90
>90
>90
>90
Asc I GGCGCGCC
A GGCGCGCC T
TT GGCGCGCC AA >90
>90
>90
>90
>90
>90
Ava I C CCCGGG G
CC CCCGGG GG
TCC CCCGGG GGA
50
>90
>90
>90
>90
>90
BamH I C GGATCC G
10
>90
>90
25
>90
>90
Bgl II C AGATCT G
GA AGATCT TC
75
25
>90
>90
BssH II G GCGCGC C
AG GCGCGC CT
TTG GCGCGC CAA
50
>90
BstE II G GGT(A/T)ACC C010
BstX I AACTGCAGAA CCAATGCATTGG AAAACTGCAG CCAATGCATTGG AA CTGCAGAA CCAATGCATTGG ATGCAT 25
25
50
>90
Cla I C ATCGAT G
G ATCGAT C
汉阙CC ATCGAT GG
CCC ATCGAT GGG
>90
50
>90
50
CG GAATTC CG
CCG GAATTC CGG >90 >90
>90
>90
>90
>90
Hae III GG GGCC CC AGC GGCC GCT TTGC GGCC GCAA >90 >90qibozi
>90
>90
>90
>90
Hind III C AAGCTT G CC AAGCTT GG
CCC AAGCTT GGG
10
75
Kpn I G GGTACC C GG GGTACC CC
CGG GGTACC CCG
>90
>90
>90
>90
Mlu I G ACGCGT C
CG ACGCGT CG
25
50
Nco I C CCATGG G CATG CCATGG CATG 50
Nde I C CATATG G
CC CATATG GG
CGC CATATG GCG
GGGTTT CATATG AAACCC
GGAATTC CATATG GAATTCC GGGAATTC CATATG GAATTCCC
75
75
>90
>90
Nhe I G GCTAGC C
CG GCTAGC CG
CTA GCTAGC TAG
10
10
25
50
Not I TT GCGGCCGC AA
ATTT GCGGCCGC TTTA
AAATAT GCGGCCGC TATAAA ATAAGAAT GCGGCCGC TAAACTAT AAGGAAAAAA GCGGCCGC AAAAGGAAAA 10
10
25
25
塞西莉亚10
10
90
>90
Nsi I TGC ATGCAT GCA
CCA ATGCAT TGGTTCTGCAGTT
10
>90
>90
Pac I TTAATTAA
routemap
G TTAATTAA C
CC TTAATTAA GG 0
25
>90
Pme I GTTTAAAC促进剂ns
G GTTTAAAC C
GG GTTTAAAC CC
AGCTTT GTTTAAAC GGCGCGCCGG 75
25
50
>90
Pst I G CTGCAG C
TGCA CTGCAG TGCA
AA CTGCAG AACCAATGCATTGG AAAA CTGCAG CCAATGCATTGGAA CTGCAG AACCAATGCATTGGATGCAT 10
>90
>90
10
>90
>90
Pvu I C CGATCG G
AT CGATCG AT
TCG CGATCG CGA
10
25
10
Sac I C GAGCTC G1010
Sac II G CCGCGG C
TCC CCGCGG GGA
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