HY5‐215
The Arabidopsis HY5 gene encodes a bZIP protein that regulates stimulus‐induced development of root and hypocotyl, Genes Dev. 1997 Nov 15; 11(22): 2983–2995.
In the genome of hy5‐215, the splicing acceptor site of the first intron (=G) was replaced by A, suggesting that this mutation causes aberrant RNA processing
In hy5‐215, the nucleotide g‐1117 (white letter), which is the last nucleotide in the first intron, is replaced by an a
HY5‐215的突变位点与野生型相比，并没有酶切位点的变化。引物在有一个错配位点的情况下，可以产生一个新的酶切位点PsiI，从而将野生型、杂合、纯合进行区分。 Design proper primers and choose proper a enzyme by dCAPS Finder 2.0
(helix.wustl.edu/dcaps/dcaps.html)
HY5proF GAGAGAATATGCGAGTGAATGAC Len 22 TM 54
HY5proR TCTAAAGTCTCTTTTATGTTTTA T A Len 25 TM 50.8
胶带机
PsiI:
但是，实验室并没有PsiI ，所以只能再去寻新的内切酶。在设计的引物有2 个错配位点时，可以产生新的酶切位点，AluI 将野生型切断。
kcvHY5-215 F CGTATCTCCTCATCGCTTTCAATAG Len 25 TM 60.0
HY5-215 R GTCCCGCTCTTTTCCTCTTTATC Len 23 TM 60.8
AluI:十年体
高强钢MYCT
The Arabidopsis bHLH Transcription Factors MYC3 and MYC4 Are Targets of JAZ Repressors and A
ct Additively with MYC2 in the Activation of Jasmonate Responses, Plant Cell. 2011 Feb; 23(2): 701–715.
MYC2
Mutagen : T‐DNA insertion
王维凝
Insertion Flanking
Sequence:
TAAAACCGCCGGAGAATCAGATCACTCCGATCTAGAAGCT
(Length:40)
根据T‐DNA PrimerDesign（signal.salk.edu/tdnaprimers.2.html）设计引物
PRODUCT_SIZE 1186
Myc2LP TGGTTTTTCTTGGTTTCGATG Len 21 TM 59.96
王驹Myc2RP CTCTAATCATTGCGTCCCAAC Len 21 TM 59.58
LBb1.3 ATTTTGCCGATTTCGGAAC BP+RP_PRODUCT_SIZE 558‐858
MYC3
Mutagen : T‐DNA insertion
myc3 F AAGGTGGGTTGTTGAAATCTAATG Len 24 TM 58.3
myc3 R GTTTTCTCCGACTTTCGTCATCA Len 23 TM 61
T-DNA ATATTGACCATCATACTCATTGC Len 23 TM 55.2
MYC4
Mutagen : T‐DNA insertion
myc4 F TCTCTCACAACTTGATCCAGCTAA Len 24 TM 60.0
myc4 R TAACCGATTACCATCTCAACCAA Len 23 TM 59.2
T-DNA ATATTGACCATCATACTCATTGC Len 23 TM 55.2
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