PROTEASE | COMPANY | Protease Capture |
Thrombin (凝血酶) Leu-Val-Pro-Arg▼Gly-Ser L-V-P-R▼G-S | Amersham-Biosciences, Novagen, SIGMA, Roche | Benzamidine-Agarose |
Factor Xa (Xa因子) Ile-Glu/Asp-Gly-Arg▼? I-E/D-G-R▼ | 阻垢剂评价Amersham-Biosciences, New England Biolabs, Roche | Benzamidine-Agarose |
Enterokinase (肠激酶) Asp-Asp-Asp-Asp-Lys▼ D-D-D-D-K▼ | New England Biolabs, Novagen, Roche | Trypsin Inhibitor-Agarose |
TEV protease (烟草蚀纹病毒蛋白酶) Glu-Asn-Leu-Tyr-Phe-Gln▼Gly E-N-L-Y–回程误差F-Q▼G | Invitrogen – Life Technologies | Ni-NTA (6His recomb. TEV) |
PreScission (PreScission 蛋白酶) Leu-Glu-Val-Leu-Phe-Gln▼Gly-Pro L-E-V-L-F-Q▼G-P | Amersham-Biosciences | GSTrap for GST fusion enzyme |
TAGZyme (标记酶) His-tag removal by Exoproteolytic Digestion | 利比亚 石油Qiagen | Ni-NTA (6His recomb. enzyme) |
Intein Site (内含肽) dithiothreitol cleavage(二硫苏糖醇清除) | New England Biolabs | DTT elimination by dialysis?(透析) |
HRV 3C Protease(3c蛋白酶) Leu-Glu-Val-Leu-Phe-Gln▼Gly-Pro L-E-V-L-F-Q▼G-P | Novagen | Ni-NTA (6His recomb. enzyme) |
SUMO Protease recognize the tertiary structure of the ubiquitin-like (UBL) protein, SUMO | Invitrogen – Life Technologies, LifeSensors | Ni-NTA (6His recomb. enzyme) |
Kex-2 -Arg-X-Lys/Arg-Arg▼ | Invitrogen – Life Technologies, | 天津信息港Ni-NTA (6His recomb. enzyme) KEX2对arg的专一性高,要求最重要。 Arg前为lys效率最高,不切-Arg-lys,Pro影响KEX2切割 |
羧肽酶 | 羧肽酶B可以切割C端的Lys或Arg;羧肽酶A可以切割C端除了Lys、Arg、Pro的氨基酸,但如果倒数第二个氨基酸为Pro两种羧肽酶均不能作用 | |
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