DRS
Life cycle of Rickettsia slovaca in L929 cell line
studied by quantitative real-time PCR and transmission electron microscopy 期刊名称： FEMS Microbiology Letters
作者： Vojtech Boldi,Jasna trus,Elena Kocianová,Magda Tuek-nidari,Katarína
tefanidesová,Katarína Schwarzová,Marcela Kúdelová,Zuzana Sekeyová,Eva
yangzhenningpitalská
年份： 2009年氢致开裂
与毒共舞关键词： Rickettsia slovaca ;quantitative real-time PCR;transmission electron
热膨胀系数microscopy;L929 cells
摘要：Rickettsia slovaca , a member of spotted fever rickettsiae, is an agent of a mild human disease known as Tibola or Debonel. Using quantitative real-time PCR we identified the highest point of multipli
cation of wild and standard type (strain B) of R. slovaca on the second vs. the fourth day postinfection. Comparing both types of R. slovaca by transmission electron microscopy substantiated different cytopathological and morphological changes in infected cells and other differences, for example a slight shift of stages during the life cycle that presented in a variety of forms and localization of the studied Rickettsia within the infected cells. Our study provides a valuable insight into the pathogenicity and virulence of R. slovaca .
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