墨鎏堕登奎兰苎主堕塞兰兰垡鲨苎
Retroviralvectorshavebecomethemostimportanttoolsforefficienttransferofgenesintoeukaryoticcells.IRES(internalribosomeentrysites)vectorspermitmultipleproteinstobeproducedfromasinglevector,asinglemRNAistranscribe-bedunderthecontrolofanupstreampromoter,andtwoormoregene
相对标准偏差productsaretranslatedindependentlyfromasinglemRNA.Thetranslationofone
活性氟化钾
IRES,andgeneiscap·dependent.andothersaretranslatedundercontrolofthe
avoidthepotentialofpromotersuppression.IRESvectorshavesignificant
雏鹰争章网
advantagesincludingasmallergenome,higherviraltilerandincreasedstabilityof
study,twoIRESretroviralvectors—pbGHlmdrandphGM-geneexpression.Inthis
CSFItkwereconstructedbygenerecombinationutilizingthemdrlgeneortheHSV-tkgeneasselectablemarkers.ThroughinvitrogenetransfectiontoestablishfibroblastcelllineswhichexpressbGHandhGM-CSEOurresultssubstantiatetheresearchofinvNocelltransplantation,exogenousgeneexpressioncontrolandcombinationgenetherapy.
ParthConstructionofrecombinantbovinegrowthhormoneIRESretroviralvectoranditsexpressioninNIH3T3cellandhumanembryolungfibroblastscell2BS
青山事件
Object:ToestablishIRESretroviralvectortoCO—expressdrug.selectable
markermdrlgenewithbovinegrowthhormone(bGH)geneinmouse
celllineNIH3T3andhumanembryolungfibroblastscell2BS
throughinvitro
7