水苏糖合成酶基因重组载体的构建及转化农杆菌作者：姜丹 姜述君 王智慧 蒲丽君 白云 方铭国发2012 2号文件
来源：《安徽农学通报》财经杂志2013年第14期 摘 绝对湿度by可有可无要：以黄瓜叶片中提取的总RNA为模板，经PCR扩增得到长度为光栅方程
1 275bp的基因片断，经过酶切测序之后，将目的基因连接到表达载体，构建成重组质粒。再利用液氮冻融法将表达载体转化农杆菌感受态。 关键词：水苏糖合成酶基因；载体构建；转化农杆菌
中图分类号 Q789 文献标识码 A 文章编号 1007-7731（2013）14-20-03
Construction of Stachyose Synthase Gene Vector and Transformation to Agrobacterium
Jiang Dan et al.
工程设计学报 （Heilongjiang Bayi Agriculture University，Collge of Life，Daqing 163319，China）
Abstract：安娜卡列尼娜论文STS gene of 1 275bp length from Cucumis sativus were cloned by RT-PCR，and were connected with cloned-vector. After sequencing and comparing with the gene showed on Genbank，absolutely accurate gene sequence which was amplied with two peculiar primier and primestar enzyme were inserted into expression vector。 STS recombined plasmid were successfully constructed，then were transformed to Agrobacterium.
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