建立超高效液相谱串联质谱法（UPLCMS/MS）同时测定首乌藤中二苯乙烯（二苯乙烯苷、虎杖苷、白藜芦醇）、蒽醌（大黄素、大黄素甲醚、大黄素8βD葡萄糖苷、芦荟大黄素）、黄酮（表儿茶素、芦丁、金丝桃苷、紫云英苷、槲皮素）及酚酸（没食子酸）等13种成分的方法。采用Waters BEH C18（21 mm×100 mm，17 μm）谱柱，流动相为水（含01%甲酸）乙腈，梯度洗脱，柱温35 风洞实验室
℃，流速025 mL·min-1，采用电喷雾离子源（ESI），多反应离子监测（MRM）扫描方式进行检测。采用TOPSIS法对13种成分进行分析评价。结果13种成分在一定浓度范围内均呈良好的线性关系，相关系数大于0991 5，精密度、重复性和稳定性良好；加样回收率在9524%～1023%，RSD均小于5%。TOPSIS分析显示广州产的样品综合质量较好。所建立的方法准确、专属性、重复性较好，可用于首乌藤中多元功效物质或指标成分的同时测定，为首乌藤药材内在质量的综合评价和全面控制提供新的方法参考。李水明
标签：首乌藤；UPLCMS/MS；二苯乙烯类；蒽醌；黄酮；酚酸；同时测定
正佳广场溜冰[Abstract]A comprehensive analytical method based on UPLCMS/MS was developed for th
e simultaneous determination of thirteen components including three stilbenes （stilbeneglucoside， polydatin， resveratrol）， four anthraquinones （emodin， physcion， emodin8βDglucopyranoside， aloeemodin）， five flavonoids （epicatechin， rutin， hyperoside， astragalin，quercetin） and one phenolic acid （gallic acid） in Polygoni Multifori CaulisThe separation was carried out on a Waters BEH C18 column（21 mm×100 mm，17 μm）with gradient elution of acetonitrilewater （01% acetic acid） at a flow rate of 025 mL·min-1， and column temperature was 35 ℃ The target compounds were analyzed by multiple reaction monitoring （MRM） mode TOPSIS analysis ware performed to evaluate the samples from different areas and commercial herbs according to the contents of thirteen components The correlation coefficients of all the calibration curves were higher than 0991 5 The average recoveries ranged from 9524% to 1023%， and the relative standard deviations were less than 5% The result of TOPSIS analysis showed that the comprehensive quality of Polygoni Multifori Caulis sample from Guangzhou was better The developed method with good repeatability and accuracy was suitable for the simultaneous determination of multiple fun
血浆分离器
ctional substances， which provided a new basis for the comprehensive assessment and overall control of the quality of Polygoni Multifori Caulis
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