b 农药学学报 2020, 22(6): 1008-1018
Chinese Journal of P esticide Science •研究论文• doi: 10.16801/j.issn. 1008-7303.2020.0146 吴淑春巴韩剑众*虞淼■章虎S 钱鸣蓉豪杰超级解霸2000
(1.浙江工商大学食品与生物工程学院,杭州310018: 2.杭州医学院,杭州310053; 3.浙江省农产品质量监督检验
测试中心,杭州310020; 4.浙江省农业科学院农产品质量标准研究所,杭州310021)
摘 要:采用高效液相谱-串联质谱(HPLC-MS/MS)手性谱柱法定量分析肝微粒体中的糠菌 。坐,通过密度泛函理论计算光谱与振动圆二光谱(VCD)和红外光谱(IR)比对,确定了糠菌 哇4种对映体的绝对构型;以大鼠、小鼠、兔、狗和人肝微粒体为模型,研究了糠菌哇的立体 选择性降解行为。结果表明:在供试肝微粒体中,4种异构体的降解均遵循一级反应动力学方 程,且在人和小鼠肝微粒体中的代谢速率相对较慢。(2S, 47?)-和(2R, 4»■糠菌哇在5种供试肝 微粒体中的立体选择性趋势一致,而(27?, 47?)- 和(2S, 4R-糠菌口坐只在兔肝微粒体中的降解有显 著的立体选择性差异,在小鼠肝微粒体中几乎没有立体选择性。酶促反应动力学结果也证实了 糠菌哇代谢的立体选择性,并显示(2R, 47?)-和(2S, 4S)-糠菌哇在供试肝微粒体中的酶促反应趋 势存在种属差异。
关键词:糠菌。坐;对映体;肝微粒体;选择性降解;种属差异中图分类号:0657.7; S481.1 文献标志码:A 文章编号:1008-7303(2020)06-1008-11Stereoselective metabolism of bromuconazole in liver microsomes from
心理学家研究发现
CHFrat, mouse, rabbit, dog and human
WU Shuchun 1,2, HAN Jianzhong 1, YU Miao 3, ZHANG Hu 4, QIAN Mingrong*4新疆大学学报
三里庵派出所
第四范式(1. College of F ood Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China; 2. Hangzhou Medical College, Hangzhou 310053, China; 3. Agricultural Products Quality Supervision and Testing Center in Zhejiang Province, Hangzhou 310020, China;4. Institute of Q uality and Standard on Agricultural Produhbcts, Zhejiang Academy of A gricultural Sciences, Hangzhou 310021, China)Abstract: Bromuconazole is a potent triazole fungicide and consists of two pairs of enantiomers
[trans(2R, 47?)-, trans(2S, 4S)-configurations and cis(2R, 45), cis(2S, 47?)-configurations]. The degradation of the enantiomers of (27?S, 4RS)- and (27?S, 4S7?)-bromuconazole by phase I metabolism was investigated using rat, mouse, rabbit, dog and human liver microsomes. Bromuconazole isomers were quantified using HPLC ・MS/MS (EST) after the separation on a combination of a reversed phase and a chiral analytical column. Moreover, the absolute configuration of four enantiomers had been determined based on the comparisons of the vibrational circular dichroism experimental spectra with the theoretical curve obtained by density functional theory calculations. The degradation of all four isomers 收稿日期:2020-03-21;录用日期:2020-05-21.
基金项目:国家自然科学基金(21507114);杭州医学院自然科学基金(2016XZA01).作者简介:吴淑春,女,副教授,研究方向为卫生检验、农产品质量与安全,E-mail : ************; •钱鸣蓉,通信作者(Author for correspondence).女,研究员,研究方向为药物残留分析,E-mail : **************