Cell Biology d201 | |||
ATCC® Number: | CRL-9591™ | Price: | $294.00 |
Designations: | MV-4-11 | Depositors: | Wistar Institute |
Biosafety Level: | 1 | Shipped: | frozen |
| 李忠仁 Medium & Serum: | See Propagation | Growth Properties: | suspension |
Organism: | Homo sapiens (human) | Morphology: | lymphoblast |
Source: | Organ: peripheral blood Disease: biphenotypic B myelomonocytic leukemia | ||
Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
This material is cited in a U.S. and/or other Patent or Patent Application, and may not be used to infringe on the patent claims. | |||
Related Cell Culture Products | |||
Applications: | transfection host(technology from amaxa) | ||
Antigen Expression: | CD4; Homo sapiens CD10; Homo sapiens CD15, human; Homo sapiens CD4 (40-96%); CD10 (4-11%); CD15 (96-99%) | ||
DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 13 D16S539: 11,12 D7S820: 8,9 THO1: 8,9.3 TPOX: 8,11 vWA: 14,15 | ||
Cytogenetic Analysis: | 48, XY, t(4;11)(q21;q23), +8, +19 | ||
Age: | 10 years | ||
Gender: | male | ||
Comments: | The MV4-11 cell line was established by Rovera and associates from the blast cells of a 10-year-old male with biphenotypic B-myelomonocytic leukemia. The growth factor, granulocyte/macrophage colony-stimulating factor (GM-CSF), was required to establish this cell line and growth factors are necessary for its continuous proliferation in chemically defined medium [PubMed: 3496132]. However, this line can be propagated in medium supplemented with 10% FBS without the addition of growth factors. IL-3 can independently support the long-term growth of this cell line but IL-3 antagonized the proliferation of MV4-11 cells in the presence of GM-CSF when both factors were used at very low concentrations. Granulocyte colony stimulating factor (G-CSF) synergized with GM-CSF in inducing proliferation of MV4-11 cells; G-CSF alone causes a transient stimulation of the cell line. Over 96% of these cells are positive by indirect immunofluorescence for the myelomonocytic antigen CD15, 40-96% are positive for the monocytic antigen CD4, and 4-11% are positive for CD10 [PubMed: 3500218]. This line was formerly designated ATCC HTB-189. | ||
Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C Growth Conditions: If the cells are maintained in a serum-free medium, it is necessary to add the following: 0.005 mg/ml transferrin, 0.005 mg/ml insulin, and 5 ng/ml GM-CSF. | ||
Subculturing: | Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml. Medium Renewal: Every 2 to 3 days | ||
Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase | ||
References: | 22617: Lange B, et al. Growth factor requirements of childhood acute leukemia: establishment of GM-CSF-dependent cell lines. Blood 70: 192-199, 1987. PubMed: 3496132 23467: Santoli D, et al. Synergistic and antagonistic effects of recombinant human interleukin (IL) 3, IL-1 alpha, granulocyte and macrophage colony-stimulating factors (G-CSF and M-CSF) on the growth of GM-CSF-dependent leukemic cell lines. J. Immunol. 139: 3348-3354, 1987. PubMed: 3500218 | ||
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