st西轴有机锡化合物摘要:
目的：筛选并鉴定胃癌circRNA表达谱，分析circRNA的生物学功能。
方法：体外培养人低分化胃癌细胞MGC-803、人中分化胃癌细胞SGC-7901、人高分化胃癌细胞NCI-N87和人正常胃上皮细胞GES-1，利用circRNA基因芯片技术检测胃癌细胞和正常胃黏膜上皮细胞中差异表达的circRNAs，利用qRT-PCR技术对部分差异表达的circRNAs进行验证，使用Cytoscape软件构建circRNA-miRNA-mRNA网络图，对下游靶基因进行GO分析和KEGG Pathway分析。 结果：与人正常胃黏膜上皮细胞相比，在不同分化程度的人胃癌细胞中，表达上调的circRNA为79个，表达下调的circRNA为229个。PCR 结果提示hsa_circRNA_103571在正常细胞中明显高于肿瘤细胞，GO分析提示hsa_circRNA_103571间接调控的靶基因在分子功能方面，主要集中在结合蛋白、调节蛋白激酶活性等方面；在细胞组分方面，主要集中在细胞内细胞器方面；在生物学过程方面，主要集中在参与细胞蛋白质修饰等方面。KEGG Pathway分析提示hsa_circRNA_103571可能通过Ras信号通路、FoxO信号通路、Hippo信号通路以及mTOR信号通路参与胃癌发生发展。 王朝云
结论：在胃癌细胞中，hsa_circRNA_103571是抑癌基因，其机制可能是与hsa-miR-16-5p相互作用，通过Ras信号通路、Hippo信号通路等多条信号通路参与胃癌的发生发展。
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关键词：环状RNA；胃癌细胞；基因芯片II
T HE EXPRESSION PROFILE AND FUNCTIONAL ANALYSIS
OF CIRCULAR RNA S IN G ASTRIC CANCER
Zhi Long（Internal Medicines）
Directed by Prof.Guo Qing Li
海南建设国际旅游岛Abstract
Objectives:To screen and identify the expression profile of circular RNA in gastric cancer cells and analyze its biological function.
Methods:Human poorly differentiated gastric cancer cells MGC-803, human moderately differentiated gastric cancer cells SGC-7901,human highly differentiated gastric cancer cells NCI-N87and human normal gastric epithelial cells GES-1are cultured in vitro.The differentially expressed
circRNAs in gastric cancer cells and normal gastric epithelial cells were detected by circRNA gene chip technology.Some differentially expressed circRNAs are validated by qRT-PCR.Cytoscape software is used to construct circRNA-microRNA-RNA network map,GO analysis and KEGG Pathway analysis are performed on downstream target genes.
Results:Compared with normal gastric epithelial cells,79up-regulated circRNAs and229down-regulated circRNAs were expressed in human gastric cancer cells with different differentiation degrees.The results of PCR indicated that hsa_circRNA_103571was significantly higher in normal cells than in cancer cells.GO analysis indicated that the target genes indirectly regulated by hsa_circRNA_103571mainly focused on binding proteins and
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regulating the activity of protein kinase,cell components mainly concentrated on organelles,biological processes mainly involved in protein modification. KEGG Pathway analysis suggests that hsa_circRNA_103571may participate in the development of gastric cancer through Ras signaling pathway,FoxO signaling pathway,Hippo signaling pathway and mTOR signaling pathway.
Conclusions:In gastric cancer cells,hsa_circRNA_103571is a tumor suppressor gene,and its mechan
ism may be to interact with hsa-miR-16-5p, and participate in tumor development through multiple signaling pathways such as Ras signaling pathway and Hippo signaling pathway.
Keywords:circular RNA gastric cancer cells gene chip
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主要缩略语英文索引
缩略词英文全称中文全称
GC Gastric cancer胃癌
FBS Fetal Bovine Serum胎牛血清
PBS Phosphate buffered saline磷酸盐缓冲液
cDNA Complementary DNA互补DNA
ceRNA Competing endongeneous RNA竞争性内源RNA circRNA Circular RNA环状RNA
MRE MicroRNA response element miRNA反应元件
ncRNA Noncoding RNA非编码RNA
LncRNA Long Noncoding RNA长链非编码RNA网格化管理
KEGG Kyoto Encyclopedia of Genes and Genomes京都基因与基因组百科全书GO Gene Ontology基因本体论
CC Cellular component细胞组成
BP Biological process生物过程
硫酸铵沉淀MF Molecular function分子功能
qPCR Quantitative real-time PCR实时荧光定量PCR
OD Optical Density光密度值
Ct Cycle threshold循环阈值
Rnase Ribonuclease核糖核酸酶
siRNA small interfering RNA小干扰RNA
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