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Table 7.1A Molecular Probes affinity-purified anti–mouse IgG conjugates, which are raised against the IgG heavy and light chains from mouse.
Cat #
Label
Emission Color
Abs *
Em *
Goat Anti–Mouse IgG, Whole Antibody Cross-Adsorbed against Human IgG and Serum
A11045
Alexa Fluor 350
Blue
346
442
M10991
Marina Blue
Blue
365
460
C962
Cascade Blue
Blue
400
420
P31583
Pacific Orange
Orange
400
551
A31553
Alexa Fluor 405
Blue
402
421
P10993
Pacific Blue
Blue
410
455
A11063
Alexa Fluor 430
Yellow-green
434
539
F2761
Fluorescein
Green
494
518
A11001
Alexa Fluor 488
Green
495
519
O6380
Oregon Green 488
Green
496
524
D31590
DyeMer 488/605
Red-orange
502
611
D31591
DyeMer 488/615
Red
502
617
D31592
DyeMer 488/630
Far-red
502
630
A31554
Alexa Fluor 500
Green
503
525
B2752
BODIPY FL
Green
505
513
O6383
Oregon Green 514
Green
511
530
A31555
Alexa Fluor 514
Yellow-green
518
540
A11002
Alexa Fluor 532
Yellow
531
554
T2762
Tetramethylrhodamine
Orange
555
580
A11003
Alexa Fluor 546
Orange
556
573
A21422
Alexa Fluor 555
Orange
555
565
P852
R-Phycoerythrin (R-PE)
Orange
496, 546, 565
578
A20980
Alexa Fluor 610–R-PE
Red
496, 546, 565
628
A20990
Alexa Fluor 647–R-PE
Far-red
496, 546, 565
668
A20983
Alexa Fluor 680–R-PE
Far-red
496, 546, 565
702
R6393
Rhodamine Red-X
Red-orange
570
590
A11004
Alexa Fluor 568
Red-orange
578
603
A11005
Alexa Fluor 594
Red
590
617
T862
Texas Red
Red
595
615
T6390
Texas Red-X
Red
595
615
A31550
Alexa Fluor 610
Red
612
628
A21050
Alexa Fluor 633
Far-red
632
647
A31574
Alexa Fluor 635
Far-red
633
647
A865
Allophycocyanin
Far-red
650
660
A21000
Alexa Fluor 680–allophycocyanin
Far-red
650
702
A21006
Alexa Fluor 750–allophycocyanin
Infrared
650
775
A21235
Alexa Fluor 647
Far-red
650
668
A21054
Alexa Fluor 660
Far-red
663
690
A21057
Alexa Fluor 680
Far-red
679
702
A21036
Alexa Fluor 700
Infrared
702
723
A21037
Alexa Fluor 750
Infrared
749
775
B2763
Biotin
NA
<300
NA
D20690
DSB-X biotin
NA
<300
NA
G21060
Alkaline phosphatase
NA
NA
NA
G21040
Horseradish peroxidase
NA
NA
NA
G31567
-Lactamase
NA
NA
NA
Goat Anti–Mouse IgG, Whole Antibody Highly Cross-Adsorbed against Bovine, Goat, Rabbit, Rat and Human IgG and Serum
P31586
Pacific Orange
Orange
400
551
P31582
Pacific Blue
Blue
410
455
A11029
Alexa Fluor 488
Green
495
519
O11033
Oregon Green 488
Green
496
524
A11030
Alexa Fluor 546
Orange
556
573
A21424
Alexa Fluor 555
Orange
555
565
A11031
Alexa Fluor 568
Red-orange
578
603
A11032
Alexa Fluor 594
Red
590
617
A21052
Alexa Fluor 633
Far-red
632
647
A31575
Alexa Fluor 635
Far-red
633
647
A21236
Alexa Fluor 647
Far-red
650
668
A21055
Alexa Fluor 660
Far-red
663
690
A21058
Alexa Fluor 680
Far-red
679
702
D20691
DSB-X biotin
NA
<300
NA
Goat Anti–Mouse IgG, F(ab')2 fragments Cross-Adsorbed against Human IgG and Serum
A11068
Alexa Fluor 350
Blue
346
442
P31585
Pacific Orange
Orange
400
551
P31581
Pacific Blue
Blue
410
455
F11021
Fluorescein
Green
494
518
A11017
Alexa Fluor 488
Green
495
519
A11018
Alexa Fluor 546
Orange
556
573
A21425
Alexa Fluor 555
Orange
555
565
A11019
Alexa Fluor 568
Red-orange
578
603
A11020
Alexa Fluor 594
Red
590
617
A21053
Alexa Fluor 633
Far-red
632
647
A21059
Alexa Fluor 680
Far-red
679
702
B11027
Biotin
NA
<300
NA
D20692
DSB-X biotin
NA
<300
NA
F21452
Alkaline phosphatase
NA
NA
NA
F21453
Horseradish peroxidase
NA
灵敏度特异度
NA
NA
Rabbit Anti–Mouse IgG, Whole Antibody Cross-Adsorbed against Human Serum
A21062
Alexa Fluor 350
Blue
346
442
A11059
Alexa Fluor 488
Green
495
519
A11060
Alexa Fluor 546
Orange
556
573
A21427
Alexa Fluor 555
Orange
555
565
A11061
Alexa Fluor 568
Red-orange
578
603
A11062
Alexa Fluor 594
Red
590
617
A21063
Alexa Fluor 633
Far-red
632
647
A21064
Alexa Fluor 660
Far-red
663
690
A21065
Alexa Fluor 680
Far-red
核冬天679
702
R21455
Horseradish peroxidase
NA
NA
NA
Chicken Anti–Mouse IgG
A21200
Alexa Fluor 488
Green
495
519
A21201
Alexa Fluor 594
Red
590
617
A21463
Alexa Fluor 647
Far-red
650
668
Donkey Anti–Mouse IgG, Whole Antibody Shows Minimum Crossreactivity to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins
A21202
Alexa Fluor 488
Green
495
519
A31570
Alexa Fluor 555
Orange
555
565
A21203
Alexa Fluor 594
Red
590
617
A31571
Alexa Fluor 647
Far-red
650
668
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm. NA = Not applicable.
  Table 7.1B Molecular Probes' affinity-purified anti–rabbit IgG conjugates, which are raised against the IgG heavy and light chains from rabbit.
Cat #
Label
Emission Color
Abs *
Em *
Goat Anti–Rabbit IgG, Whole Antibody Cross-Adsorbed against Human IgG and Serum, Mouse IgG and Serum, and Bovine Serum
A11046
Alexa Fluor 350
Blue
346
442
M10992
Marina Blue
Blue
365
460
C2764
Cascade Blue
Blue
400
420
P31584
Pacific Orange
Orange
400
551
A31556
Alexa Fluor 405
Blue
402
421
P10994
Pacific Blue
Blue
410
455
A11064
Alexa Fluor 430
Yellow-green
434
539
F2765
Fluorescein
Green
494
518
A11008
Alexa Fluor 488
Green
495
519
O6381
Oregon Green 488
Green
496
524
D31600
DyeMer 488/605
Red-orange
502
611
D31601
DyeMer 488/615
Red
502
617
D31602
DyeMer 488/630
Far-red
502
630
A31557
Alexa Fluor 500
Green
503
525
B2766
BODIPY FL
Green
505
513
A31558
Alexa Fluor 514
Yellow-green
518
540
A11009
Alexa Fluor 532
Yellow
531
554
T2769
Tetramethylrhodamine
Orange
555
580
A11010
Alexa Fluor 546
Orange
556
573
A21428
Alexa Fluor 555
Orange
555
565
P2771MP
R-Phycoerythrin (R-PE)
Orange
496, 546, 565
578
A20981
Alexa Fluor 610–R-PE
Red
496, 546, 565
628
A20991
Alexa Fluor 647–R-PE
Far-red
496, 546, 565
668
A20984
Alexa Fluor 680–R-PE
Far-red
496, 546, 565
702
R6394
Rhodamine Red-X
Red-orange
570
590
A11011
Alexa Fluor 568
Red-orange
578
603
A11012
Alexa Fluor 594
Red
590
617
T2767
Texas Red
Red
595
615
T6391
Texas Red-X
Red
595
615
A31551
Alexa Fluor 610
Red
612
628
A21070
Alexa Fluor 633
Far-red
632
647
A31576
Alexa Fluor 635
Far-red
633
647
A10931
Allophycocyanin
Far-red
650
660
A21001MP
Alexa Fluor 680–allophycocyanin
Far-red
650
702
A21244
Alexa Fluor 647
Far-red
650
668
A21073
Alexa Fluor 660
Far-red
663
690
A21076
Alexa Fluor 680
Far-red
679
702
A21038
Alexa Fluor 700
Infrared巴洛克音乐下载
702
723
A21039
Alexa Fluor 750
Infrared
749
775
B2770
Biotin
NA
<300
NA
D20694
DSB-X biotin
NA
<300
NA
G21079
Alkaline phosphatase
NA
NA
NA
G21234
Horseradish peroxidase
NA
NA
NA
G31568
-Lactamase
NA
NA
NA
Goat Anti–Rabbit IgG, Whole Antibody Highly Cross-Adsorbed against Bovine, Goat, Human, Mouse and Rat IgG
A21068
Alexa Fluor 350
Blue
346
442
A11034
Alexa Fluor 488
Green
495
519
O11038
Oregon Green 488
Green
496
524
A11035
Alexa Fluor 546
Orange
556
573
A21429
Alexa Fluor 555
Orange
555
565
A11036
Alexa Fluor 568
Red-orange
578
603
A11037
Alexa Fluor 594
Red
590
617
A21071
Alexa Fluor 633
Far-red
632
647
A31577
Alexa Fluor 635
Far-red
633
647
A21074
Alexa Fluor 660
Far-red
663
690
A21109
Alexa Fluor 680
Far-red
679
702
D20695
DSB-X biotin
NA
<300
NA
Goat Anti–Rabbit IgG, F(ab')2 fragments Cross-Adsorbed against Human Serum
A11069
Alexa Fluor 350
Blue
346
442
A11070
Alexa Fluor 488
Green
495
519
A11071
Alexa Fluor 546
Orange
556
573
A21429
Alexa Fluor 555
Orange
555
565
A21069
Alexa Fluor 568
Red-orange
578
603
小冰期
A11072
Alexa Fluor 594
Red
590
617
A21072
Alexa Fluor 633
Far-red
632
647
A21075
Alexa Fluor 660
Far-red
663
690
A21077
Alexa Fluor 680
Far-red
679
702
D20696
DSB-X biotin
NA
<300
NA
F21456
Alkaline phosphatase
NA
NA
NA
Chicken Anti–Rabbit IgG
A21441
Alexa Fluor 488
Green
495
519
A21442
Alexa Fluor 594
Red
590
617
A21443
Alexa Fluor 647
Far-red
650
668
Donkey Anti–Rabbit IgG, Whole Antibody Shows Minimum Crossreactivity to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins
A21206
Alexa Fluor 488
Green
495
519
A31572
Alexa Fluor 555
Orange
555
565
A21207
Alexa Fluor 594
Red
590
617
A31573
Alexa Fluor 647
Far-red
650
668
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm. NA = Not applicable.
Table 7.1C Molecular Probes' affinity-purified anti–rat IgG conjugates, which are raised against the IgG heavy and light chains from rat.
Cat #
Label
Emission Color
Abs *
Em *
Goat Anti–Rat IgG, Whole Antibody Cross-Adsorbed against Mouse IgG and Serum
A21093
Alexa Fluor 350
Blue
346
442
A11006
Alexa Fluor 488
Green
495
519
O6382
Oregon Green 488
Green
496
524
A11081
Alexa Fluor 546
Orange
556
573
A21434
Alexa Fluor 555
Orange
555
565
A11077
Alexa Fluor 568
Red-orange
578
603
A11007
Alexa Fluor 594
Red
590
617
T6392
Texas Red-X
Red
595
615
A21094
Alexa Fluor 633
Far-red
632
647
A21247
Alexa Fluor 647
Far-red
650
668
A21095
Alexa Fluor 660
Far-red
663
690
A21096
Alexa Fluor 680
Far-red
679
702
Chicken Anti–Rat IgG
A21470
Alexa Fluor 488
Green
495
519
A21471
Alexa Fluor 594
Red
590
617
A21472
Alexa Fluor 647
Far-red
650
668
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm.
Table 7.1D Molecular Probes' affinity-purified anti–human IgG conjugates, which are raised against the IgG heavy and light chains from human.
Cat #
Label
Emission Color
Abs *
Em *
Goat Anti–Human IgG, Whole Antibody Cross-Adsorbed against Mouse, Rabbit and Bovine Sera
A11013
Alexa Fluor 488
Green
495
519
A21089
Alexa Fluor 546
Orange
556
573
A21433
Alexa Fluor 555
Orange
555
565
A21090
Alexa Fluor 568
Red-orange
578
603
A11014
Alexa Fluor 594
Red
590
617
A21091
Alexa Fluor 633
Far-red
632
647
A21445
Alexa Fluor 647
Far-red
650
668
D20700
DSB-X biotin
NA
<300
NA
Chicken Anti–Human IgG
A21464
Alexa Fluor 488
Green
495
519
Rabbit Anti–Human IgG
A21218
Alexa Fluor 488
Green
495
519
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm. NA = Not applicable.
Table 7.2E Molecular Probes' affinity-purified anti–sheep IgG conjugates, which are raised against the IgG heavy and light chains from sheep.
Cat #
Label
Emission Color
Abs *
Em *
Donkey Anti–Sheep IgG, Whole Antibody Cross-Adsorbed against Mouse, Rabbit, Bovine and Human Serum and Human IgG
A21097
Alexa Fluor 350
Blue
346
442
F2810
Fluorescein
Green
494
518
A11015
Alexa Fluor 488
Green
495
519
A21098
Alexa Fluor 546
Orange
556
573
A21436
Alexa Fluor 555
Orange
555
565
A21099
Alexa Fluor 568
Red-orange
578
603
A11016
Alexa Fluor 594
Red
590
617
A21100
Alexa Fluor 633
Far-red
632
647
A21448
Alexa Fluor 647
Far-red
650
668
A21101MP
Alexa Fluor 660
Far-red
663
690
A21102
Alexa Fluor 680
Far-red
679
702
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm.
Table 7.1F Molecular Probes' affinity-purified anti–goat IgG conjugates, which are raised against the IgG heavy and light chains from goat.
Cat #
Label
Emission Color
Abs *
Em *
Donkey Anti–Goat IgG, Whole Antibody Cross-Adsorbed against Human, Mouse, Rabbit and Rat IgG
A21081
Alexa Fluor 350
Blue
346
442
A11055
Alexa Fluor 488
Green
495
519
A11056
Alexa Fluor 546
Orange
556
573
A21432
Alexa Fluor 555
Orange
555
565
A11057
Alexa Fluor 568
Red-orange
578
603
A11058
Alexa Fluor 594
Red
590
617
A21082
Alexa Fluor 633
Far-red
632
647
A21447
Alexa Fluor 647
Far-red
650
668
A21083
Alexa Fluor 660
Far-red
663
690
A21084
Alexa Fluor 680
Far-red
679
702
Rabbit Anti–Goat IgG, Whole Antibody Cross-Adsorbed against Human and Rat IgG
A11078
Alexa Fluor 488
Green
495
519
A21085
Alexa Fluor 546
Orange
556
573
A21431
Alexa Fluor 555
Orange
555
565
A11079
Alexa Fluor 568
Red-orange
578
603
A11080
Alexa Fluor 594
Red
590
617
A21086
Alexa Fluor 633
Far-red
632
647
A21446
Alexa Fluor 647
Far-red
650
668
A21073
Alexa Fluor 660
Far-red
663
690
A21088
Alexa Fluor 680
Far-red
679
702
R21458
Alkaline phosphatase
NA
NA
NA
R21459
Horseradish peroxidase
NA
NA
NA
Chicken Anti–Goat IgG
A21467
Alexa Fluor 488
Green
495
519
A21468
Alexa Fluor 594
Red
590
617
A21469
Alexa Fluor 647
Far-red
650
668
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm. NA = Not applicable.
Table 7.2G Molecular Probes affinity-purified anti–chicken IgG conjugates, which are raised against the IgG heavy and light chains from chicken.
Cat #
Label
Emission Color
Abs *
Em *
Goat Anti–Chicken IgG, Whole Antibody
A11039
Alexa Fluor 488
Green
495
519
A11040
Alexa Fluor 546
Orange
556
573
A21437
Alexa Fluor 555
Orange
555
565
A11041
Alexa Fluor 568
Red-orange
578
603
A11042
Alexa Fluor 594
Red
590
617
A21103
Alexa Fluor 633
Far-red
632
647
A21449
Alexa Fluor 647
Far-red
650
668
A21104
Alexa Fluor 660
Far-red
663
690
D20701
DSB-X biotin
NA
<300
NA
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm. NA = Not applicable.
Table 7.1H Molecular Probes' affinity-purified anti–guinea pig IgG conjugates, which are raised against the IgG heavy and light chains from guinea pig.
Cat #
Label
Emission Color
Abs *
Em *
Goat Anti–Guinea Pig IgG, Whole Antibody Highly Cross-Adsorbed against Bovine, Chicken, Goat, Hamster, Human, Mouse, Rabbit, Rat and Sheep Sera
A11073
Alexa Fluor 488
Green
495
519
A11074
Alexa Fluor 546
Orange
556
573
A21435
Alexa Fluor 555
Orange
555
565
A11075
Alexa Fluor 568
Red-orange
578
603
A11076
Alexa Fluor 594
Red
590
617
A21105
Alexa Fluor 633
Far-red
632
647
A21450
Alexa Fluor 647
Far-red
650
668
A21106
Alexa Fluor 660
Far-red
663
690
* Absorption (Abs) and fluorescence emission (Em) maxima of conjugates in nm.
Table 7.2I Molecular Probes' affinity-purified goat anti-IgM * antibody conjugates, which are raised against the IgM µ chain.
Fluorophore
Abs/Em 
Anti-Mouse
Anti-Rat
Anti-Human
Alexa Fluor 350
346/442
A31552
 
 
Alexa Fluor 488
495/519
A21042
A21212
A21215
Alexa Fluor 546
556/573
A21045
 
 
Alexa Fluor 555
555/565
A21426
 
 
Alexa Fluor 568
578/603
A21043
 
 
Alexa Fluor 594
590/617
A21044
A21213
A21216
Alexa Fluor 633
632/647
A21046
 
 
Alexa Fluor 647
650/668
A21238
A21248
A21249
Alexa Fluor 660
663/690
A21047
 
 
Alexa Fluor 680
679/702
A21048
 
 
DSB-X biotin
NA
D20693
 
 
* May also react with IgM from other species.
 Absorption (Abs) and fluorescence emission (Em) maxima of conjugates, in nm. NA = Not applicable.
Table 7.1J Molecular Probes' affinity-purified goat anti–hamster IgG antibodies.
Cat #
Label
Emission Color
Abs *
Em *
Goat Anti–Hamster IgG, Whole Antibody Cross-Adsorbed against Mouse and Rat IgG
A21110
Alexa Fluor 488
Green
495
519
A21111
Alexa Fluor 546
Orange
556
573
A21112
Alexa Fluor 568
Red-orange
578
603
A21113
Alexa Fluor 594
Red
590
617
A21114
Alexa Fluor 633
Far-red
632
647
A21451
Alexa Fluor 647
Far-red
650
668
* Approximate absorption (Abs) and emission (Em) maxima of conjugates, in nm.

Table 7.1 — Summary of Molecular Probes' secondary antibody conjugates
pvdf
Antibody
Host
Blue Emission
Green Emission
Yellow Emission
Orange Emission
Red-Orange Emission
Red Emission
Far-Red Emission
Infrared Emission
Biotin
DSB-X biotin
Alkaline Phosphatase
Horseradish Peroxidase
-Lactamase
Anti–mouse IgG
Goat
Anti–mouse IgM
Goat
 
 
 
 
 
 
Anti–rabbit IgG
Goat
Anti–rat IgG
Goat
 
 
 
 
 
 
Anti–rat IgM
Goat
 
 
 
 
 
 
 
 
 
 
Anti–guinea pig IgG
Goat
 
 
 
 
 
 
 
 
Anti–chicken IgG
Goat
 
 
 
 
 
 
 
Anti–hamster IgG
Goat
 
 
 
 
 
 
 
 
Anti–human IgG
Goat
 
 
 
 
 
 
 
Anti–human IgM
Goat
 
 
 
 
 
 
 
 
 
 
Anti–goat IgG
Donkey
 
 
 
 
 
 
Anti–mouse IgG
Donkey
 
 
 
 
 
 
 
 
 
Anti–rabbit IgG
Donkey
 
 
 
 
 
 
 
 
 
Anti–rat IgG
Donkey
 
 
 
 
 
 
 
 
 
 
 
Anti–sheep IgG
Donkey
 
 
 
 
 
 
Anti–goat IgG
Rabbit
 
 
 
 
 
 
Antibody
Host
Blue Emission
Green Emission
Yellow Emission
Orange Emission
Red-Orange Emission
Red Emission
Far-Red Emission
Infrared Emission
Biotin
DSB-X biotin
Alkaline Phosphatase
Horseradish Peroxidase
-Lactamase
Anti–mouse IgG
Rabbit
 
 
 
 
 
Anti–human IgG
Rabbit
 
 
 
 
 
 
 
 
 
 
 
 
Anti–rat IgG
Rabbit
 
 
 
 
 
 
 
 
 
 
 
Anti–mouse IgG
Chicken
 
 
 
 
 
 
 
 
 
 
Anti–human IgG
Chicken
 
 
 
 
 
 
 
 
 
 
 
 
Anti–rat IgG
Chicken
 
 
 
 
 
 
 
 
 
 
Anti–goat IgG
Chicken
 
 
 
 
 
 
 
 
 
 
Anti–rabbit IgG
Chicken
 
 
 
 
 
 
 
 
 
 

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