常⽤载体测序引物列表载体名称正向引物反向引物
pACT T7 T3
pACT2 GAL4 AD(5’AD) 3'AD/pACT2-R PACYCDUET-1(MCSII) DuetUP2 T7TER
pAD/pl-DEST CMV-F
pAD-Track pAD-Track-F ⽆
pAS2-1 5’BD M13-26/48 PB42AD PB42ADF PB42ADR PBAD PBAD-F PBAD-R pBABE pBABE5’ pBABE3’ PBACPAK8 BAC1
BAC2 pBK-CMV T7/M13-20 T3/M13-26 PBS(SK/KS)/M13- M13F/T7
T3/M13R pBV220 PBV220F PBV220R pBI121
需根据酶切位点确认需根据酶切位点确认 pCAMBIA 1301(1300) 需根据酶切位点确认需根据酶切位点确认 pCAMBIA 2300 需根据酶切位点确认需根据酶切位点确认
神洁巾PCANTB5E S1/M13R S6
pCAT3-enhancer pGL3+
pcDNA3.0 pEGFP-N5/T7 SP6/BGH
pcDNA3.1 pEGFP-N5/T7 BGH
pcDNA4 T7/pEGFP-N5 BGH
pcDNA6 T7/pEGFP-N5 BGH
联想et60pcDNAII T7
SP6 pCE2.1 M13F
M13R pCEP4 pCMV5R/PCEP-F pEGFP-N5/EBV-R pCF-T M13F
爱英优选M13R pCI
T7（17Base ） pCMV5R pCI-neo T7（17Base ） T3
pCMS-EGFP T7
T3 pCMV-3Tag-4A T3 /pFlag-CMV-F T7
pCMV5 pCMV5F pCMV5R pCMV5-Flag pCMV5F
pCMV5R pCMV-MYC/NUC pCMV5F
pCMV5R PCMV-HA pCMV5F
pCMV5R pCMV-Sport
SP6/M13R
pCMV-Tag T3
T7 pCMV-TNT T7/SP6
pCMV5R pCR2.1-TOPO M13F/T7
M13R pCR3.1 T7
BGH pCS2 SP6
T7
PDNR-LIB M13F M13R
pDonar M13F M13R pDONR201 pDONR-F pDONR-R pDONR221 M13F M13R
pDrive T7 SP6 pDRIveR T7 SP6
pDsRED1-C1 pDsRED-C1-F pECFP-C-3 pDsRed1-N1 pEGFP-N-5 RFP-Nrev
pDsRed-Monomer-N1 pEGFP-N-5 pDsRed1-N
pDsRED2-C1 pDsRED-ex-C1-F pEGFP-N-3’
pEGFP-N-5 pDsRed1-N pDSRED2-N1
pDSRED-N1 pEGFP-N-5’ PDSRED-N-R pEASY-BLUNT M13F/T7 M13R
Ter
pEASY-E1 T7 T7
pEASY-T1 M13F/T7 M13R
pEASY-T3 M13F/T7 M13R/SP6 pECFP-C pEGFP-C-5’ pECFP-C-3' pECFP-N1 pEGFP-N-5’ pEGFP-N-3’
pEF/myc/ER pEF-F pCDNA3.1R pEGFP-C pEGFP-C-5’ pEGFP-C-3' pEGFP-N pEGFP-N-5’ pEGFP-N-3’ pENTR/D-TOPO M13F M13R
Ter
pET-*(His) T7 T7
pET22(a,b,c) T7 T7
Ter
Ter
pET28,30,24,49 T7 T7
Ter
pET32(a,b,c) T7/S.tag T7
pET-42a (-b, -c)(+) Stag T7 Ter
广西来宾一中
pET50 T7/s.tag T7TER pET44 T7/s.tag T7
Ter pEYFP-N1 pEGFP-N-5’ pEGFP-N-3’ PETBlue M13-20 PETBlueDOWN PETDUET-1(MCSI) PBRrevBam DuetDOMN1 PETDUET-1(MCSII) DuetUP2 T7TER
pEYFP-C1 pEGFP-C-5’ pEGFP-C-3' pEYFP-N1 pEGFP-N-5’ pEGFP-N-3’ pFastBac pFastBac-F pFastBac-R pFastBac HT_A,B,C pFastBac-PH pECFP-C-3 pFastBac1 pFastBac-PH pECFP-C-3 pFastBac Dual (MCSI) pFastBac-PH pECFP-C-3 pFLAG-CMV CMV30/pFLAG-CMV-F CMV-24/pFLAG-CMV-R
p3xFlag-CMV-7.1 CMV-F/CMV24 CMV30
pGAD424 5’AD 3'AD
pGADGH 5’AD 3'AD
pGADT7-Rec 5’AD/T7 3'AD
pGAPZa-A/B/C a-FACTOR 3'AOX
forward 3'AOX
pGAPZ-A/B/C pGAP
pGBKT7 T7 3'BD
pGEM-T(-Easy) M13F/T7 SP6/M13R
pGEX-(*)T pGEX-5’ pGEX-3’
pGL2 pCMV5R PGL3-
Pgl3-BASIC pGL3+ PGL3-
pGL3-Enhancer pGL3+ PGL3-
PinPoint TM PinPoint Primer SP6
pIRES PIRES-F/T7 T3/PIRES-R奇圣胶囊
pIRES2-EGFP pIRES2-EGFP.P5’ pIRES2-EGFP.P3’ pLenti6/v5-Dest pEGFP-N-5’
PLEXA PLEXA-F PLEXA-R PLNCX PLNCX-F PLNCX-R pLXSN pLXSN-F pLXSN-R
Pjc1-tac T7
归化城
pJet.1.21.Blunt T7
primer M13F(-47) pMAL-c2E MalE
P3/PMAL-C2X-R pMAL-C2x P5/PMAL-C2X-F
primer M13F(-47) pMAL-p2X MalE
PMD18-T M13R(-48) M13F(-47) PMD19-T M13F(-47) M13R(-48) pMIR-report M13F
PPC86 PPC86-F PPC86-R
3’AOX
pPIC9K 5’AOX/a-factor
3'AOX pPICZa 5'AOX/PPICZa-F
pPROEXHTA M13R(-48)
pQE30or40 pQE30+ pQE30- pRECEIVER CMV-F
pRSET T7 T7TER PRSFDUET-1(MCSI) DuetUP1 DuetDOWN1 PRSFDUET-1(MCSII) DuetUP2 T7TER pShuttle-CMV pShuttle-CMV-F pShuttle-CMV-R PSILENCE-1.0-U6 T7 T3
pSilencer3.1-H1 hygro M13F(-47) 3.0rev
pSILENCEV2.0-U6 T7 2.0rev
pSK01-T M13F M13R
pSK-CMV T7 T3
Psos H1
PSP64/65 SP6
pSP72 T7 SP6
T7/M13R
pSport1 SP6/M13F(-47)
pSTBlue-1 T7 SP6
pSUPER T7 T3
pT7Blue(R) T7 M13F(-47)
pTA2 M13F/T7 T3/M13R
/T7 M13R
pT-Adv M13F
pTARGET TM pTarget-F/T7 arget-R
PTHIOHISA,B,C TRX-FORWARD TRX-REVERSE pTO-T7 T7 T7TER
PBV220-/PTRC99C-R pTRC99a-c PQE30+/PTRC99C-F pTriPLEx2 PT5/5'TriplEx2 T7
pTz57r/t M13+ M13-
pTWIN-1 T7 T7TER
pUC18(19)/118(119) M13F(-47) M13R(-48)
M13F(-47)/M13F pUC57 M13R(-48)/M13R
pUCm-T M13F/T7 M13R
pVAX1 T7 BGH
T3/M13R/M13R(-48) pWSK29 T7/M13F/M13F(-47)
pXT7 T7 SP6
pYES2 T7 pYes2.R
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