王子伦理
摘要
通信系统概论目的 观察多西紫杉醇诱导hepg2 PC3 MD-231 A549 四种肿瘤细胞凋零并对其作用机制进行探讨。
方法 培养4种细胞，取对数生长期细胞分为五组，空白对照组、模型组、蜂胶黄酮高、中、低剂量组，剂量分别为200mg/L、100 mg/L、50 mg/L.药物预处理2h后，做MTT检测。通过实验数据计算IC50.
结果 各组细胞IC50值
结论 多西紫杉醇对肿瘤细胞有诱导凋亡作用
关键词 多西紫杉醇赤道银行；hepg2；A549；PC3；M-231；；细胞凋亡
Abstract
Study on the effect of docetaxel on tumor cell proliferation inhibition and in tumor chemotherapy, take the cell count, morphologic observation, flow cytometry method for the detection and observation respectively and combined with docetaxel on proliferation of human tumor cell lines in nude mice bearing human tumor inhibition; to establish animal model, to treat separately and combined with docetaxel, and blank control. Observation of growth and pathological characteristics of the tumor body. Through the experiments of MTT detection test of docetaxel on tumor cell proliferation inhibition. Using different concentrations of tumor cell suppression test, the influence of the concentration on the survival of tumor cells.The experiment is divided into two parts, respectively, and MTT detection of cell culture.周涵 成都书店
海关总署公告2012年第15号Keywords: Docetaxe l Stem cells Research Application
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