目的 构建含PRDX5基因启动子的荧光素酶报告质粒。方法 ①通过生物信息学方法,查询PRDX5基因启动子核酸序列;②使用PCR扩增人PRDX5基因启动子片段,将启动子插入到pGL3-Basic载体中,构建含PRDX5基因启动子片段的荧光素酶报告质粒;③双酶切及测序确定PRDX5基因启动子的荧光素酶报告质粒扩增正确。结果 PCR扩增的PRDX5启动子片段插入到载体中,经测序证实正确。结论 成功构建含PRDX5启动子的荧光素酶报告质粒。 Abstract:ObjectiveTo construct a pGL3-PRDX5-promoter-Luc plasmid and to vertifying its luciferase activity. Methods①search the nucleic acid sequence of the promoter PRDX5 by bioinformatics analysis.pgl3②Methods of PCR was performed to amplify the promoter PRDX5. the promoter PRDX5 was inserted into pGL3-Basic cloning vector to construct luciferase reporter plasmid with the promoter PRDX5.③pGL3-PRDX5-promoter-Luc plasmid was verified by double-enzyme cleavage and sequencing method.ResultspGL3-PRDX5-promoter-Luc plasmid was identified to be correct by double-enzyme cleavage and sequencing method.Conclusion A pGL3-PRDX5-promoter-Luc plasmid was successfully constructed.