pEGM-7ZF(+)
北京华越洋生物VECT5370 pEGM-‐7ZF(+)
pEGM-‐7ZF(+)载体基本信息
载体名称: pEGM-‐7ZF(+)
高拷贝/低拷贝: -‐-‐
启动子: -‐-‐
载体大小: 3000bp
5' 测序引物及序列: -‐-‐
3' 测序引物及序列: -‐-‐差速防坠器
载体标签: -‐-‐
载体抗性: Ampicillin
筛选标记: -‐-‐
备注: -‐-‐
稳定性: -‐-‐
组成型: -‐-‐
病毒/非病毒: 非病毒
pEGM-‐7ZF(+)载体质粒图谱和多克隆位点信息
pEGM-‐7ZF(+)载体简介
The p GEM-‐7Zf(+) V ector(a) i s a d erivative o f t he p GEM?-‐3Zf(+) V ector(a) a nd c ontains t he origin o f r eplication o f t he f ilamentous p hage f1. T he p lasmid s erves a s a s tandard c loning vector, as a template for in vitro transcription, and it can be used for the production of circular ssDNA. The plasmid contains SP6 and T7 RNA polymerase promoters flanking a region of multiple cloning sites within the α-‐peptide coding region of β-‐galactosidase (1). Insertional inactivation of the α-‐peptide allows recombinant clones to be directly identified by color screening on indicator plates. The multiple cloning region is unique and includes restriction sites for Apa I, Aat II, Sph I, Xba I, Xho I, EcoR I, Kpn I, Sma I, Csp45 I, Cla I, Hind III, BamH I, Sac I, BstX I and Nsi I. This arrangement is designed specifically for generation of unidirectional deletions with the Erase-‐a-‐Base? System(b). The polylinker contains restriction enzyme sites that produce 5′ overhangs or blunt ends (sensitive to Exonuclease III) flanked on both sides by blocks of restriction sites that generate 3′ overhangs (resistant to Exonuclease III). For induction of ssDNA, ba
cterial cells containing pGEM?-‐7Zf(+) recombinants are infected with an appropriate helper phage. The plasmid then enters the f1 replication mode, and the resulting ssDNA is exported from the cell as an encapsidated viruslike particle. The sequence of the ssDNA rescued upon infection with helper phage is complementary to the sequence shown in Figure 1. The exported ssDNA can be used for mutagenesis in vitro or can be sequenced
多肽药物合成using t he T7 P romoter P rimer o r p UC/M13 F orward P rimer.电热地膜
pEGM-‐7ZF(+)载体序列
ORIGIN
1 GGGCGAATTG GGCCCGACGT CGCATGCTCC TCTAGACTCG AGGAATTCGG TACCCCGGGT 61 TCGAAATCGA TAAGCTTGGA TCCGGAGAGC TCCCAACGCG TTGGATGCAT AGCTTGAGTA 121 TTCTATAGTG TCACCTAAAT AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA 181 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT 241 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC 301 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCG
G CCAACGCGCG GGGAGAGGCG 361 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC 421 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG 481 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA 541 AGGCCGCGTT GCTGGCGTTT TTCGATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC 601 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC 661 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG 721 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT 781 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC 841 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC 901 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG 961 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG 1021 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA 1081 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG 1141 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT 1201 CACGTTAAGG GATTTTGGTC ATGAGATT
AT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA 1261 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT 1321 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG 1381 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA 1441 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC 1501 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT 1561 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG 1621 TTGTTGGCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA 1681 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG 1741 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA 1801 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG 1861 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATACCG CGCCCGGCGA CCGAGTTGCT 1921 CTTGCCCGGC GTCAATACGG GATAATAGTG TATGACATAG CAGAACTTTA AAAGTGCTCA 1981 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA 2041 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG 2101 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGAC
AC 2161 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT 2221 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC 2281 CGCGCACATT TCCCCGAAAA GTGCCACCTG TATGCGGTGT GAAATACCGC ACAGATGCGT 2341 AAGGAGAAAA TACCGCATCA GGCGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG
2401 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG 2461 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG 2521 GGCTCCCTTT AGGGTTCCGA TTTAGAGCTT TACGGCACCT CGACCGCAAA AAACTTGATT 2581 TGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT 2641 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA 2701 TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA 2761 ATGAGCTGAT TTAACAAATA TTTAACGCGA ATTTTAACAA AATATTAACG TTTACAATTT 2821 CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT 2881 ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG 2941 GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAA TTGTAATACG ACTCACTATA
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其他大肠杆菌表达载体：
pBV221 ptdTomato pET-52b(+) pAmCyan
pDsRed-Express2 pBV220 pCold-GST pColdS-SUMO
pCold TF pCold IV pCold III pCold II
pCold I pE-SUMO pCold-ProS2 pBAD102/D-TOPO
pBAD202/D-TOPO pACYC184 pBAD/Thio-TOPO pBad/Myc-His C
pBad/Myc-His B pBad/Myc-His A pBad/His C pBad/His B
pBad/His A pBAD-TOPO pET-23b(+) pET-23a(+)
pET-23c(+) pET-23(+) pET-12b(+) pET-12c(+)
阀门专机pET-12a(+) pET-11b(+) pET-11a(+) pET-11c(+)
pBad24 pQE-82L pQE-81L pQE-80L
pQE-32 pQE-9 pQE-16 pQE-31
pQE-60 pQE-70 pQE-40 pET-51b(+)
pET-50b(+) pET-49b(+) pET-48b(+) pET-47b(+)
pET-26b(+) pET-32a(+) pET-21b(+) pET-22b(+)
pET-14b pET-16b pET-15b pET-19b
pET-20b(+) pET-21d(+) pET-21c(+) pET-21b(+)
pET-21a(+) pET-24a(+) pET-24d(+) pET-25b(+)
pET-27b(+) pET-28a(+) pET-30a(+) pET-42a(+)
pET-43.1c(+) pET-43.1b(+) pET-43.1a(+) pET-44a(+)
pET-44c(+) pET-46 EK/LIC pET-37b(+) pTrcHis2 C
pTrcHis2 B pTrcHis2 A pET303/CT-His pET302/NT-His pRSET-CFP pRSET-EmGFP pRSET-BFP pGFPuv
pET300/NT-DEST pET301/CT-DEST pGEM-T pBad43
pGEX-4T-3 pGEX-5X-2 pBlueScript SK(+) pG-Tf2
硅酸盐水泥熟料pG-KJE8 pGro7 pET-SUMO pSE380
pET-17b pET102/D-TOPO pCDFDuet-1 pMAL-p5x
pTf16 pET-28c(+) pBluescript II SK(+) pET-30b(+)
pSUMO pProEX HTc pProEX HTb pProEX HTa
pKD3 pKD13 pKD46 pTYB1
pTYB2 pTWIN2 pBluescript II KS(-) pTYB12
pMAL-p5e pACYCDuet-1 pEGM-11ZF(+) pEGM-7ZF(+) PinPoint Xa-3 PinPoint Xa-2 PinPoint Xa-1 pSP73
pSP64 pTWIN1 pTYB11 pTXB1
pET-5b(+) pBad/gIII C pBad/gIII B pBad/gIII A pET-5a(+) pMal-p4X pMal-p2G pkk223-3
pkk232-8 pCYB1 pEZZ18 pBAD18
pMAL-c5x pMal-p2E pMal-p2X pET-44 EK/LIC pET-43.1 EK/LIC pET-41 EK/LIC pMal-c4X pTrcHis B
pET-31b(+) pET-3b(+) pET-41a(+) pGEX-3X
pGEX-4T-2 pETDuet-1 pGEX-4T-1 pTrc99a
pET-28b(+) pET-His pALEX a,b,c pACYC177
pBR322 pKD4 pKD20 pMXB10壁炉门
pEcoli-6xHN-GFPuv pKJE7 pRSET B pGEX-KG
pGEX-2T pRSFDuet-1 pCOLADuet-1 pTrcHis C pTrcHis A pET-41b(+) pET-42b(+) pET-3a(+) pGEX-6P-3 pGEX-6P-2 pGEX-6P-1 pGEX-5X-3 pGEX-5X-1 pGEX-2TK pRSET A pMal-c2G
pMal-c2E pMal-c2X pRSET C pQE-30
pET-45b(+) pET-44b(+) pET-42c(+) pET-41c(+) pET-40b(+) pET-33b(+) pET-39b(+) pET-32 EK/LIC pET-32 Xa/LIC pET-32c(+) pET-32b(+) pET-30 Xa/LIC pET-30 EK/LIC pET-30c(+) pET-29c(+) pET-29b(+) pET-29a(+) pET-24c(+) pET-24b(+) pET-24(+)
pET-23d(+) pET-11d(+) pBad33
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