远程调压阀3种木材DNA提取方法比较研究作者：余敏 魏宇创 张浩 刘盛全来源：《安徽农学通报》2021年第03期 沟槽式管接头
母鸡接鸡蛋
翻模机 摘 要：以微凹黄檀木材标本为研究对象，分别采用BATB法、PTB法和SDS法对微凹黄檀不同部位木材进行DNA提取试验，并扩增DNA条形码序列ITS2、trnH-psbA和matK，分析不同DNA提取方法对微凹黄檀木材DNA提取和DNA条形码序列扩增的影响。结果表明：采用BATB法从微凹黄檀木材心、边材组织中提取的DNA浓度以及DNA条形码扩增效率均要高于其他2种方法，说明BATB法更适合从长期存储木材中提取DNA。 关键词：微凹黄檀;DNA提取;DNA条形码;木材识别
中图分类号 S781.1 文献标识码 A 文章编号 1007-7731（2021）03-0006-04
Comparison of Three Methods of DNA Extraction from Wood
YU Min et al.
（School of Forestry & Landscape Architecture， Anhui Agricultural University， Hefei 230036，China; Key Lab of State Forest and Grassland Administration on “Wood Quality Improvement & High Efficient”， Hefei 230036， China）
清理废旧钢筋泵 Abstract：The xylarium wood specimens of Dalbergia retusa were selected from the Wood Collection. Three methods， i.e.， BTAB， PTB and SDS were employed to extract DNA in sapwood and heartwood for further PCR amplification of three potential DNA barcode sequences （ITS2， trnH-psbA， and matK）. The effects of different methods on DNA extraction and PCR for target sequences were analyzed. The results showed that the concentration of DNA and efficacy of PCR using BTAB method was higher than that of PTB and SDS methods from sapwood and heartwood. The BTAB method is more suitable for extracting DNA from long-stored wood.
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