目录
缩略语表 (1)
英文摘要 (2)
中文摘要 (5)
论文正文Kartogenin复合海藻酸盐凝胶促进大鼠前交叉韧带重建后腱-骨愈合的研究 (7) 第一章前言 (7)乙酰正丙醇
1.1 选题缘由 (7)
1.2 研究背景 (7)
1.3 研究意义 (8)
第二章大鼠冈上肌腱止点生长发育的组织形态学观察 (10)
2.1 材料与方法 (10)
2.2 结果 (14)
2.3 讨论 (16)
洗肾机
第三章SD大鼠膝关节前交叉韧带重建的小动物模型构建及组织学观察 (18)
3.1 材料与方法 (18)
3.2 结果 (25)
3.3 讨论 (28)
第四章观察Kartogenin复合海藻酸盐缓释凝胶对大鼠膝前交叉韧带重建术后腱-骨愈合的影响 (29)
4.1 材料与方法 (29)
4.2 结果 (32)
4.3 讨论 (35)
全文总结 (37)
参考文献 (38)
文献综述促进前交叉韧带重建后腱-骨愈合方法的研究进展 (42)
参考文献 (48)
攻读硕士学位期间的学术成果 (53)
致谢 (54)
缩略语表
英文缩写英文全称中文全称
ACL Anterior cruciate ligament 前交叉韧带
BMP Bone morphogenetic protein 骨形态发生蛋白BMSCs Bone marrow mesenchymal stem cells 骨髓间充质干细胞
Cm Centimeter 厘米
铝塑型材ColⅠCollagen typeⅠⅠ型胶原
ColⅡCollagen typeⅡⅡ型胶原
ColⅩCollagen typeⅩⅩ型胶原
d Day 天
ECM Extracellular matrix 细胞外基质
EDTA Ethylenediamine tetra-acetic 乙二胺四乙酸二钠
HE Hematoxylin-eosin 苏木精-伊红染
Kg Kilogram 千克
L Litre 升
Micro-CT Micro-computed tomography 微计算机断层扫描技术µg Microgram 微克
min Minute 分钟
ml Milliliter 毫升
MMPs Matrix metalloproteinases 基质金属蛋白酶
Mol Mole 摩尔
MSCs Mesenchymal stem cells 间充质干细胞
PBS Phosphate buffered saline 磷酸盐缓冲液
pH Power of hydrogen 酸碱度
折弯机上模
TGF-β3Transforming growth factor-β3转化生长因子
Acceleration of tendon-bone healing in anterior cruciate ligament reconstruction using kartogenin-encapsulated感温元件
alginate gelation in rat model
Abstract
Background and Objectives:
上路床Anterior cruciate ligament (ACL) is the most frequently injured ligament of the knee and lead to about 400,000 ACL reconstructions per year worldwide. Because ACL can not heal spontaneously, surgical reconstruction using autograft or allograft tissue is frequently used to restore normal knee kinematics and to prevent degenerative osteoarthritis（OA）. ACL attaches to bone across a functionally graded interface which is called “enthesis". The typical four-layered structure of enthesis is consist of four zones (tendon, fibrocartilage, mineralized fibrocartilage, and bone). It is believed to play an important role for dissipation of stress concentrations at mature fibrocartilaginous interfaces. So, the key part of ACL reconstruction is how to regenerate normal fibrocartilaginous enthesis structure efficiently.
It has been reported that Scx+/Sox9+ progenitors contribute to the establishment of the junction between cartilage and tendon/ligament by lineage tracing. Sox9 is a high-mobility group box-containing transcription factor that functions as an important regulator of cartilage formation. Meanwhile, another study has reported that tenocytes can direct convert into chondrocytes by Sox9. All of this suggest that chondrogenic factor may play an important role in augmenting the healing of the tendon-bone interface. And what happened is that chondrogenic inducing factor, e.g BMP-2, TGF-β3, PTH have been proved to own the ability to improves tendon-bone healing.
Given cartilage importance during development, the overall objective of this dissertation was first to examine the role of cartilage in development and morphogenesis of the tendon-to-bone insertion, and then established a tendon-bone healing small animal model in ACL reconstruction and use this model to evaluate the potential therapeutic effects of chondrogenic molecular kartogenin（KGN）during enthesis healing. KGN is a small
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