MICROBIOLOGICAL ANALYSIS – LABORATORY PRACTICES SURFACE SPREAD PLATING TECHNIQUE
1 SCOPE范围
A standard method for the detection of microorganisms in liquid samples containing from 10 to 10,000 microorganisms per ml.
此标准方法用于检测液体样品中的微生物含量，其微生物含量要求在10 ~ 10,000 个/ ml钻井泥浆泵
。 2 FIELD OF APPLICATION应用范围
The technique is applicable to any type of liquid brewery sample. It may be necessary to use the sample decimal dilution technique prior to plating.
此技术可用于啤酒厂所有的液体样品，样品在倒平板前可能需用十倍稀释技术。
3 PRINCIPLE原理
The sample is spread as a thin layer on a prepoured agar growth medium which is then incubated at an appropriate temperature and under suitable conditions. During incubation, the microorganisms grow and develop into distinct colonies on the surface of the agar medium.
涂一层薄薄样品到已倒好的琼脂培养基上，这样细胞围在培养基上。在适当的温度和条件下培养，微生物在培养基上生长成明显的菌落。 4 REAGENTS试剂
Safety note:安全备注
Powdered media may cause irritation to eyes, skin and respiratory system. Do not breathe dust, wear suitable protective clothing, weigh dry components in a fume cupboard or use an appropriate mask where exposure is reasonably probable. In case of contact with eyes, rinse immediately with plenty of water. After contact with skin, wash immediately with plenty of water. If inhaled, remove to fresh air. 粉末培养基会刺激眼睛，
皮肤以及呼吸系统。不要吸入粉尘，戴适当的防护罩，在通风橱里称干成分或使用适当的面具。万一接触眼睛，用大量水冲洗。接触皮肤，立刻用水冲洗，万一吸入，转移呼吸新鲜空气。
4.1 Prepoured agar growth medium in 9 cm petri-dishes. The medium may be selective, non-selective and/or differential; but not necessarily clear.
倒琼脂培养基与直径为9 cm培养皿中。培养基可以是选择性、非选择性以及鉴定性的。不一定要求清澈。
5 APPARATUS仪器
5.1 Volumetric sterile pipettes, 0.1-1.0 ml volume (scale 0.1).无菌移液管
5.2 Bunsen Burner气体喷
5.3 Incubator, a thermostatically controlled cupboard自动恒温控制培养箱
分合闸电磁铁5.4 Thermostatically controlled water bath, or oven at 46 °C自动恒温控制水浴，或46 °C
电极臂干燥箱
5.5 Drigalsky spreader (disposable, Sigma Z376779) Drigalsky涂布工具（一次性）
6 PROCEDURE程序
6.1 The agar medium is prepared in advance. (See 02.15.03 Microbiological Media)
提前准备好琼脂培养基。
6.2 Aseptically place 0.1 or 0.2 ml of the sample for testing (or a dilution thereof) on the surface of a prepoured agar plate (in duplicate).
无菌取0.1 或0.2 ml样品(或稀释样)于平板琼脂培养基上用于检测。 做两个。
6.3 Spread the sample carefully and evenly over the surface of the medium using a Drigalsky spreader. It is important that the layer of sample is very thin, especially if the medium contains cycloheximide as this must be in close contact with the whole sample.Use a sterile spreader for each plate.
用Drigalsky涂布工具小心均匀涂布样品于培养基表面。涂的样品层要薄，这很重要。尤其是含培养基，应与样品充分接触。每个平板都用无菌涂布工具。
6.4 Allow the plate to dry for 15 minutes with the lid closed. Incubate in an inverted position at an appropriate temperature / condition for a suitable time interval.
盖紧盖子凝固15分钟，然后放在适当的温度/条件下培养一定时段。
6.5 Count the colonies which form on the surface of the medium.
从培养基表面对菌落计数。
7 EXPRESSION OF RESULTS结果表示太阳能整体浴室
Results are expressed as Colony Forming Units per ml of sample (CFU/ml).
压电陶瓷驱动器>野苹果园结果表示：CFU(菌落形成单位)/ml样品。
7.1 Calculation计算
rage number of CFU/plate x 10 x dilution factor (CFU/ml)
Sample = 0.2 ml: average number of CFU/plate x 5 x dilution factor (CFU/ml)
9 NOTES and RELATED DOCUMENTS备注与相关文件
Not applicable无
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