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蛋白酶切位点

Enzymes E.C.Number Cleavage sites Serine proteases E.C.3.4.21.

α-chymotrypsin(胰凝乳蛋白酶，糜蛋白酶)E.C.3.4.21.1Tyr-|-Xaa,Trp-|-Xaa,Phe-|-Xaa,and

also Leu-|-Xaa,Met-|-Xaa,

Trypsin(胰朊酶，胰蛋白酶) E.C.3.4.21.4Arg-|-Xaa,Lys-|-Xaa,

Pancreatic elastase(胰弹性蛋白酶) E.C.3.4.21.36Ala-|-Xaa,and also Gly-|-Xaa,

Val-|-Xaa,Ser-|-Xaa,

Thrombin(凝血酶) E.C.3.4.21.5Arg-|-Gly,

Plasmin(胞质素，胞浆素) E.C.3.4.21.7Lys-|-Xaa,>Arg-|-Xaa

Prolyl oligopeptidase,or prolyl

endopeptidase

E.C.3.4.21.26Pro-|-Xaa,>>Ala-|-Xaa

Plasma kallikrein(血浆激肽释放酶) E.C.3.4.21.34Arg-|-Xaa,Lys-|-Xaa,including

电视升降机Arg-|-Ser,Lys-|-Arg,

Leukocyte elastase(白细胞弹性蛋

白酶

),or neutrophil elastase(中性白细

胞弹性蛋白酶),or lysosomal

elastase(溶酶体弹性蛋白酶)

E.C.3.4.21.37Val-|-Xaa,Ala-|-Xaa,

Cysteine proteases E.C.3.4.22.

Cathepsin B(组织蛋白酶) E.C.3.4.22.1Arg-Arg-|-Xaa,and also Leu-|-Xaa,

Ala-|-Xaa,Phe-|-Xaa,Trp-|-Xaa,

Clostripain(梭菌蛋白酶),or endoproteinase Arg-C E.C.3.4.22.8Arg-|-Xaa including Arg-|-Pro,but not

Lys-|-Xaa

Calpain(钙激活中性蛋白酶)-1,or μ-calpain E.C.3.4.22.52Met-|-Xaa,Tyr-|-Xaa and

Arg-|-Xaa(with Leu or Val as the P2

residue)

Aspartic acid proteases E.C.3.4.23.

Pepsin(胃朊酶，胃蛋白酶) E.C.3.4.23.1Preferentially Phe-|-Xaa,Tyr-|-Xaa,

and also Leu-|-Xaa,and

Trp-|-Xaa,,ideally with Xaa=

Phe,Trp,or Tyr

Cathepsin(组织蛋白酶)D E.C.3.4.23.5Preferentially Phe-|-Xaa,Tyr-|-Xaa,

冰浆机

and also Leu-|-Xaa,ideally with Xaa

≠Ala or Val

Metalloproteases E.C.3.4.24.

Neprilysin(脑啡肽酶),or enkephalinase(脑啡肽酶),or neutral endopeptidase E.C.3.4.24.11Xaa-|-Tyr,Xaa-|-Phe,Xaa-|-Trp,and

Xaa-|-Leu,

Thimet oligopeptidase(甲拌磷寡肽酶),or endo-oligopeptidase A,or endopeptidase(肽链内切

酶)24.15,or pz-peptidease(肠促胰酶素)E.C.3.4.24.15Xaa-|-Arg,Xaa-|-Ser,Xaa-|-Ile,熏洗仪

Xaa-|-Ala,Xaa-|-Gly,

Aminopeptidases E.C.3.4.11.N-term

Leucyl-aminopeptidase E.C.3.4.11.1Preferentially Leu-|-Xaa,but not

Arg-|-Xaa,and Lys-|-Xaa,

Aminopeptidase M or N,or alanyl-aminopeptidase,or membrane alanine aminopeptidase E.C.3.4.11.2Preferentially Ala-|-Xaa,and

Tyr-|-Xaa,if Yaa-Pro-|-Xaa in term

with Yaa=Ala,Val,Leu,Ile,Phe,Tyr or硅胶加热膜

Trp,then the dipeptide Yaa-Pro could

be released

Aminopeptidase B E.C.3.4.11.6Arg-|-Xaa,Lys-|-Xaa, Aminopeptidase A,or

angiotensinase,or

glutamyl-aminopeptidase

E.C.3.4.11.7Glu-|-Xaa>>Asp-|-Xaa,

Dipeptidyl-peptidases and

tripeptidyl-peptidases

E.C.3.4.14.N-term(di-and tripeptides)

Dipeptidyl-peptidase I,or cathepsin(组织蛋白酶)C or J E.C.3.4.14.1Xaa-Yaa-|-Zaa,if Xaa≠Arg or Lys,or

Yaa≠Pro,or Zaa≠Pro

Dipeptidyl-peptidase IV E.C.3.4.14.5Preferentially Xaa-Pro-|-Zaa,(but also

Xaa-Ala-|-Yaa)with Yaa≠Pro or Hyp Prolyl tripeptyl-peptidase E.C.3.4.14.12Xaa-Yaa-Pro-|-Zaa,if Zaa≠Pro Peptidyl-Dipeptidases E.C.3.4.15.C-term

Peptidyl-depeptidase A,or angiotensin-converting enzyme E.C.3.4.15.1Xaa-|-Yaa-Zaa,if Yaa≠Pro or Zaa≠

Asp or Glu

Metallo-carboxypeptidases E.C.3.4.17.C-term

Carboxypeptidase A E.C.3.4.17.1Xaa-|-Yaa if Yaa≠Asp,Glu,Arg,Lys

or Pro

Carboxypeptidase B,or E.C.3.4.17.2Xaa-|-Arg and Xaa-|-Lys

protaminase

Carboxypeptidase N,or

Lysine(arginine),

Carboxypeptidase(羧基肽酶),or

硫酸钙晶须kininase I(激肽酶)

E.C.3.4.17.3Xaa-|-Lys>>Xaa-|-Arg Carboxypeptidase U or R E.C.3.4.17.20Xaa-|-Arg and Xaa-|-Lys

Glutamate Carboxypeptidase II,or folate hydrolase E.C.3.4.17.21Xaa-|-Glu,preferentially with Xaa=

垂直母排Asp or Glu

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