高效液相谱法测定苯妥英钠血药浓度作者：左晶包覆胶水 宋新文 汪洋 许琼 易揭膜刘炘来源：《中国医药导报》2011年第34期烷基叔丁基醚
[摘要] 目的：建立测定血清中苯妥英钠浓度的高效液相谱法。方法：以Hypersil C18柱（4.6 mm×200 mm，10 μm）为谱柱，流动相为甲醇-水（50∶50），流速为0.8 ml/min，检测波长为230 nm，血清样品加入3 μl硫酸溶液（6 M）混匀后用重蒸乙醚提取。结果：苯妥英钠血清样品线性范围为2.00～150.00 mg/L，低、中、高三种不同浓度（8.33、33.33、100.00 mg/L）加样回收率分别为98.40%、95.50%、102.71%，日内、日间RSD均小于引道结构图5%。结论：本方法准确性好，灵敏度高，操作简便，可满足苯妥英钠血药浓度监测要求。 [关键词] 苯妥英钠；血药浓度；高效液相谱法
[中图分类号] R969.4 [文献标识码] A [文章编号] 1673-7210荀果（2011）12（a）-072-02
Determination on the concentration of Phenytoin Sodium in serum by HPLC
电工工具包 ZUO Jing, SONG Xinwen, WANG Yang, XU Qiong, LIU Xin
Department of Pharmaceutical, the Children′s Hospital in Wuhan City, Hubei Province, Wuhan 430016, China
[Abstract] Objective: To establish HPLC method to determine the concentration of Phenytoin Sodium in serum. Methods: The chromatographic column was Hypersil C18 (4.6 mm×200 mm, 10 μm), the mobile phase was methanol and water (50∶50), the flow rate was 0.8 ml/min, and the detective wavelength was 230 nm. 3 μl sulphuric acid solution (6 M) was added to serum and was mixed evenly, then Phenytoin Sodium was extracted by evaporated ethylether. Results: The linear range of Phenytoin Sodium in serum was 2.00-150.00 mg/L. The recovery rates of mild, middle and high concentration (8.33, 33.33, 100.00 mg/L) were 98.40%, 95.50%, 102.71% respectively, and the within-day RSD and day to day RSD were both lower than 5%. Conclusion: The method is accurate, sensitive and simple, which can satisfy the requisition of determination of Phen
ytoin Sodium in serum.
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