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常用DNA载体

I. 常用DNA载体
1. Lambda DNA
Lambda DNA is commonly used as a substrate in restriction enzyme activity assays and for preparation of DNA molecular weight standards. The phage is isolated from a heat-inducible lysogenic E.coli W3110 ( cI 857 S am7) strain. The DNA is isolated from purified phage by standard purification procedure.
The molecule is linear double-stranded DNA, 48502 base pairs in length.
Molecular Weight: 31.5 x 106 daltons.
Storage Buffer: 10mM Tris-HCl (pH 7.6) and 1mM EDTA.
Quality Control: Gel analysis for purity and homogeneity of Alw21I, CpoI and HindIII fragmentation patterns.

编号	产品名称	浓度	包装	价格（元）
GV0201	Lambda DNA	100ng /ul	50ug	70.00

2. pBR322 DNA
pBR322 DNA is a commonly used plasmid cloning vector in E.coli , isolated from E.coli ( dam+, dcm+ ) by ion exchange chromatography. The molecule is a double-stranded circle, 4361 base pairs in length.
Molecular Weight: 2.83 x 106 daltons.
Storage Buffer: 10mM Tris-HCl (pH 7.6) and 1mM EDTA.
Quality Control: ?
· More than 90% is in the supercoiled form.
· Gel analysis for purity and homogeneity of AluI, BsuRI and HindIII fragmentation patterns.

编号	产品名称	浓度	包装	价格（元）
GV0202	pBR322 DNA	500ng /ul	25ug	180.00

3. pUC18 DNA
pUC18 DNA is a commonly used plasmid cloning vector in E.coli , isolated from E.coli ( dam+, dcm+ ) by ion exchange chromatography. The molecule is a double-stranded circle, 2686 base pairs in length.
Molecular Weight: 1.74 x 10 6 daltons.
Storage Buffer: 10mM Tris-HCl (pH 7.6) and 1mM EDTA.
Quality Control:
· More than 90% is in the supercoiled form.
· Gel analysis for purity and homogeneity of EcoR I, HindIII and Msp I fragmentation patterns.

编号	产品名称	浓度	包装	价格（元）
GV0203	PUC18 DNA	500ng /ul	25ug	210.00

4. pUC19 DNA
pUC19 DNA is a commonly used plasmid cloning vector in E.coli , isolated from E.coli ( dam+, dcm+ ) by ion exchange chromatography. The molecule is a double-stranded circle, 2686 base pairs in length.
Molecular Weight: 1.74 x 10 6 daltons.
Storage Buffer: 10mM Tris-HCl (pH 7.6) and 1mM EDTA.
Quality Control:
· More than 90% is in the supercoiled form.
· Gel analysis for purity and homogeneity of EcoR I, HindIII and Msp I fragmentation patterns.

编号	产品名称	浓度	包装	价格（元）
GV0204	PUC19 DNA	500ng /ul	25ug	210.00

5. pUC57 DNA
pUC57 DNA is a commonly used plasmid cloning vector in E.coli , isolated from E.coli ( dam+, dcm+ ) by ion exchange chromatography.
The molecule is a double-stranded circle, 2710 base pairs in length.
Molecular Weight: 1.76 x 10 6 daltons.
Storage Buffer: 10mM Tris-HCl (pH 7.6) and 1mM EDTA.
Quality Control:
· More than 90% is in the supercoiled form.
· Gel analysis for purity and homogeneity of EcoR I, HindIII and Msp I fragmentation patterns.

编号	产品名称	浓度	包装	价格（元）
GV0205	PUC57 DNA	500ng /ul	25ug	210.00

II. 其他常用载体
本系列载体均为抽干状态，每管10ug。

编号	载体名称	长度(bp)	包装	价格（元）	抗性	参考测序引物
GV0301	G-pBluescriptIISK(+)	2961	10ug	300	AmpR	M13+/M13-, T7, T3
GV0302	G-pBV220	3665	10ug	300	AmpR	pBV220+ /pBV220-
GV0303	G-pcDNA3.0	5446	10ug	500	AmpR	T7,SP6
GV0304	G-pCDNA3.1(+)	5428	10ug	500	AmpR	T7, BGH rev
GV0305	G-pCDNA3.1(-)	5427	10ug	500	AmpR	T7, BGH rev
GV0306	G-pcDNA3.1-HisA	5514	10ug	500	AmpR	T7,BGH rev
GV0307	G-pcDNA3.1(-) Hygro	5597	10ug	500	AmpR	T7,BGH rev
GV0308	G-pcDNA3.1(-) Zeo	5015	10ug	500	AmpR	T7,BGH rev
GV0309	G-pcDNA II	3013	10ug	500	AmpR	T7,SP6
GV0310	G-pCR2.1	3913	10ug	500	AmpR, KanR	M13+/M13-, T7
GV0311	pgl3G-pEGFP-C1	4731	10ug	500	KanR,NeoR	EGFP-N,EGFP-C
GV0312	G-pET-3a	4640	10ug	500	AmpR	T7, T7 ter
GV0313	G-pET-11a	5677	10ug	500	AmpR	T7, T7 ter
GV0314	G-pET-11d	5677	10ug	500	AmpR	T7, T7 ter
GV0315	G-pET14b	4671	10ug	500	AmpR	T7, T7 ter
GV0316	G-pET15b	5708	10ug	500	AmpR	T7, T7 ter
GV0317	G-pET17b	3306	10ug	500	AmpR	T7, T7 ter
GV0318	G-pET19b	5717	10ug	500	AmpR	T7, T7 ter
GV0319	G-pET21b+	5443	10ug	500	AmpR	T7, T7 ter
GV0320	G-pET22b	5493	10ug	500	AmpR	T7, T7 ter
GV0321	G-pET24a	5310	10ug	500	KanR	T7, T7 ter
GV0322	G-pET24b	5310	10ug	500	KanR	T7, T7 ter
GV0323	G-pET25b+	5547	10ug	500	AmpR	T7, T7 ter
GV0324	G-pET26b	5360	10ug	500	AmpR	T7, T7 ter
GV0325	G-pET28a	5369	10ug	500	KanR	T7, T7 ter
GV0326	G-pET29a	5371	10ug	500	KanR	T7, T7 ter
GV0327	G-pET30a	5422	10ug	500	KanR	T7, T7 ter
GV0328	G-pET31b+	5742	10ug	500	AmpR	T7, T7 ter

GV0329	G-pET32a	5900	10ug	500	AmpR	T7, T7 ter
GV0330	G-pET-44a	7311	10ug	500	AmpR	T7, T7 ter
GV0331	G-pFASTBAC	4775	10ug	500	AmpR	pFASTBAC+, pFASTBAC-
GV0332	G-pFASTBAC-HTb	4856	10ug	500	AmpR	pFASTBACh+, pFASTBACh-
GV0333	G-pFASTBAC-HTa	4856	10ug	500	AmpR	pFASTBACh+, pFASTBACh-
GV0334	G-pFASTBAC-HTc	4856	10ug	500	AmpR	pFASTBACh+, pFASTBACh-
GV0335	G-pFLAG-MAC	5071	10ug	500	AmpR	pFLAG+, pFLAG-
GV0336	G-pGEM-3Zf(+)	3197	10ug	500	AmpR	T7,SP6
GV0337	G-pGEM-5Zf	3000	10ug	500	AmpR	T7,SP6
GV0338	G-pGEM-T	3000	10ug	500	AmpR	T7,SP6
GV0339	G-pGEM-T easy*	3015	10ug	500	AmpR	M13+/M13-, T7, SP6
GV0340	G-pGEX-2T	4801	10ug	500	AmpR	pGEX5’, pGEX3’
GV0341	G-pGEX-4T-1	4580	10ug	500	AmpR	pGEX5’, pGEX3’
GV0342	G-pGEX-4T-2	4970	10ug	500	AmpR	pGEX5’, pGEX3’
GV0343	G-pGEX-5X-2	4585	10ug	500	AmpR	pGEX5’, pGEX3’
GV0344	G-pGEX-6p	4597	10ug	500	AmpR	pGEX5’, pGEX3’
GV0345	G-pGL3-basic	4818	10ug	500	AmpR	pGL3F,pGL3R
GV0346	G-pHRL-TK	4045	10ug	500	AmpR	——
GV0347	G-pIRES	6092	10ug	500	AmpR	——
GV0348	G-pIRES-Hyg	5723	10ug	500	AmpR	——
GV0349	G-pIRESHyg3	5752	10ug	500	AmpR	——
GV0350	G-pIRES2-DsRed	5264	10ug	500	KanR,NeoR	——
GV0351	G-pIRES2-EGFP	5308	10ug	500	KanR,NeoR	——
GV0352	G-pTREShyg2	5788	10ug	500	AmpR	——
GV0353	G-pLXSN	5874	10ug	500	AmpR	——
GV0354	G-pPEPTIDE	5146	10ug	1000	AmpR	——
GV0355	G-pPIC3.5K	9004	10ug	500	AmpR, KanR	5’AOX, 3’AOX
GV0356	G-pPIC9	8023	10ug	500.00	AmpR	5’AOX, 3’AOX
GV0357	G-pPIC9k	9276	10ug	500.00	AmpR, KanR	5’AOX, 3’AOX
GV0358	G-PPICZalpha A	3593	10ug	500.00	ZeoR	5’AOX, 3’AOX
GV0359	G-pQCXIH	7761	10ug	500.00	AmpR, HygR	pQCseqF, PQCseqR,
GV0360	G-pQE30	3461	10ug	500.00	AmpR	pQE5’, pQE3’
GV0361	G-pQE30-XA	3509	10ug	500.00	AmpR	pQE5’, pQE3’
GV0362	G-pQE70	3426	10ug	500.00	AmpR	pQE5’, pQE3’
GV0363	G-pRSET-A	2897	10ug	500.00	AmpR	T7,pRSET rev
GV0364	G-pRSET-B	2897	10ug	500.00	AmpR	T7,pRSET rev
GV0365	G-pRSET-C	2897	10ug	500.00	AmpR	T7,pRSET rev
GV0366	G-pTriEX-3-Neo	6445	10ug	500.00	AmpR, NeoR	TriEXUP, TriEXDOWN
GV0367	G-pTriEx-4	5238	10ug	500.00	AmpR	TriEXUP, TriEXDOWN
GV0368	G-pTZ19R	2862	10ug	500.00	AmpR	M13+/M13-, T7
GV0369	G-pVAX1	2999	10ug	500.00	KanR	T7,BGH

* 注：该载体为常规环状载体，不能作为TA克隆载体用

III. pTG19-T载体

pTG-19T是一种新颖的pUC系列T载体，其多克隆位点更多的单切点和b-半乳糖苷酶阅读框架的调整大大方便了克隆的蓝/白筛选。插入位点两端独特设计的两个PstI位点使插入片段可以用Pst I单酶切进行检测，也可以用非常廉价而高效的EcoR I和Hind III 双酶切进行检测。可以用M13通用引物和T7启动子引物对PCR产物进行测序。pTG-19T含有T7 RNA聚合酶的启动子，可以对插入片段进行体外转录。
详情请参见本目录“分子生物学试剂盒”部分。

编号	产品名称	浓度	包装	价格（元）
GV0101	pTG19-T vector	20ng/ul	1ug	220.00

IV. 常用菌株

菌株名称	说明	包装	价格 (元)
JM109	甘油菌，常规质粒制备的宿主菌，可进行蓝白斑筛选	0.5ml x2	100.00
DH5a	甘油菌，常规质粒制备的宿主菌，可进行蓝白斑筛选	0.5ml x2	100.00
XL1-Blue	甘油菌，常规质粒制备的宿主菌，可进行蓝白斑筛选，生长速度稍慢，容易制备感受态细胞	0.5ml x2	100.00
XL10-gold	甘油菌，常规质粒制备的宿主菌，可进行蓝白斑筛选，更适合大载体的高效转化，生长速度快	0.5ml x2	100.00
Stbl3	甘油菌，常规质粒制备的宿主菌，可进行蓝白斑筛选，对于不稳定的片段克隆效果好	0.5ml x2	100.00

克隆载体和表达载体,菌株：您可以从晶美生物工程有限公司的产品目录中查到您所需要的载体的名称和图谱，也可以登陆www. jingmei. 网站，Promega的载体的名称和图谱的相关信息请登陆www. promega.网站。

本文发布于:2024-09-25 16:39:19，感谢您对本站的认可！

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