[摘要] 目的 探讨糖基化终产物(AGEs)对体外培养兔视网膜Müller细胞表达碱性成纤维细胞生长因子（bFGF）的影响。方法 体外分离并培养兔视网膜Müllercao20细胞。37℃条件下制备牛血清白蛋白AGEs（AGEs-BSA）及其对照物。应用AGEs-BSA及其对照物作用于Müller细胞，采用免疫细胞化学(ICC)方法半定量检测AGEs对视网膜Müller细胞表达大蒜破瓣机bFGF的影响。结果 与对照组相比，AGEs作用下体外培养的兔视网膜Müller细胞bFGF的表达增高(P＜0.05或 P＜0.01)，且具有一定的时间和浓度依赖性。结论 AGEs可以上调Müller细胞表达bFGF，推测AGEs可能通过增加bFGF的表达从而加速糖尿病视网膜病变(DR)新生血管的形成。
[关键词] 糖基化终产物；碱性成纤维细胞生长因子；视网膜分离式导电滑环Müller细胞；免疫细胞化学
Effect of advanced glycosylation end products on expression of basic
fibroblast growth factorin cultured rabbit retinal Müller cells
AI Jing 1,LIU Yao 1,LUAN Jie 1,CHEN Ping-sheng 2
数字模型(1.Department of Ophthalmology,Zhongda Hospital,Southeast University,Nanjing 210009,China;2.Department
of Pathology,School of Basic Medical Science,Southeast University,Nanjing 210009,China)育苗袋>HDPE多孔加筋缠绕波纹管
Abstract:Objective To investigate the effect of advanced glycosylation end products(AGEs)on expression of basic fibroblast growth factor(bFGF)in rabbit retinal Müller cells in vitro.Methods Rabbit retinal Müller cells were cultured according to the descriptions in literatures.AGEs-BSA and its control were made in 37℃ in vitro.Müller cells were divided into AGEs-BSA group,AGEs-BSA control group and blank control group.AGEs-BSA group and AGEs-BSA control group were respectively treated with 5 different concentration series of AGEs-BSA and AGEs-BSA control for1,3,6 and 9 days,while blank control group was incubated without any intervention.Then bFGF expression in Müller cells was half-quantitatively detected by immunocytochemistry(ICC).Results Comparing with blank control and AGEs-BSA control
group,AGEs-BSA evoked a time and concentration-dependent increase of bFGF expression by retinal Müller cells.Conclusion AGEs might promote the neovascularization of diabetic retinopathy through up regulating the expression of bFGF in Müller cells.
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