A liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based assay to profile 20 plasma steroids in endocrine disorders 期刊名称： Clinical Chemistry and Laboratory Medicine
海马ゆう管道内衬作者： Zhenxin Wang,Hao Wang,Yingfei Peng,Fangjun Chen,Wei Guo单相整流桥
年份： 2020年
期号： 第9期
电子
保护膜关键词： endocrine disorders;liquid chromatography-tandem mass spectrometry (LC-MS/MS);panel;pathway;steroids摘要：Background Liquid chromatography-tandem mass spectrometry (LC-
MS/MS)-based assays are employed in more and more clinical laboratories to quantify steroids. The steroid quantification by LC-MS/MS shows great value in screening or diagnosing endocrine disorders; however, the number of functional steroids included in the LC-MS/MS methods is still limited. Methods Here, we describe the performance and validation of a 20-steroid plasma panel by LC-MS/MS. The pa
虚拟墓地
cn-mnel included progestogens (including mineralocorticoids and glucocorticoids), androgens and estrogens biosynthesized in steroid metabolic pathways. The LC-MS/MS method was validated according to guidance documents, and subsequently employed to profile steroid changes in endocrine disorders. Results Using LC-MS/MS, 20 steroids were separated and quantified in 8 min.
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