pTT5
北京华越洋生物VECT6098 pTT5
pTT5载体基本信息
载体名称: pTT5
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶，多克隆位点
启动子: CMV
载体大小: 4401 bp
5' 测序引物及序列: CMV-F:CGCAAATGGGCGGTAGGCGTG 3' 测序引物及序列: --
载体标签: 无
载体抗性: 氨苄青霉素
筛选标记: --
克隆菌株: DH5α等
宿主细胞（系）: 常规细胞系（293、CV-1、CHO等）
备注: 利用该质粒构建的基因表达是非融合表达，在构建质粒时需要在基因前面添加Kozak序列来增加蛋白表达效率。 稳定性: 瞬时表达
组成型/诱导型: 诱导性
病毒/非病毒: 非病毒
pTT5载体质粒图谱和多克隆位点信息
pTT5载体简介
pTT5载体是很好的哺乳细胞瞬时蛋白表达载体，经常用于哺乳细胞的抗体轻链或者重链的表达。
pTT5载体序列
ORIGIN
1 GTACATTTAT ATTGGCTCAT GTCCAATATG ACCGCCATGT TGACATTGAT TATTGACTAG 61 TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT
121 TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC 181 GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG 241 GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG 301 TCCGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT 361 GACCTTACGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT 421 GGTGATGCGG TTTTGGCAGT ACACCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT 481 TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA 541 CTTTCCAAAA TGTCGTAATA ACCCCGCCCC GTTGACGCAA ATGGGCGGTA GGCGTGTACG 601 GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCCTCA CTCTCTTCCG 661 CATCGCTGTC TGCGAGGGCC AGCTGTTGGG CTCGCGGTTG AGGACAAACT CTTCGCGGTCpgl3
721 TTTCCAGTAC TCTTGGATCG GAAACCCGTC GGCCTCCGAA CGGTACTCCG CCACCGAGGG 781 ACCTGAGCGA GTCCGCATCG ACCGGATCGG AAAACCTCTC GAGAAAGGCG TCTAACCAGT 841 CACAGTCGCA AGGTAGGCTG AGCACCGTGG CGGGCGGCAG CGGGTGGCGG TCGGGGTTGT 901 TTCTGGCGGA GGTGCTGCTG ATGAT
GTAAT TAAAGTAGGC GGTCTTGAGA CGGCGGATGG 961 TCGAGGTGAG GTGTGGCAGG CTTGAGATCC AGCTGTTGGG GTGAGTACTC CCTCTCAAAA 1021 GCGGGCATTA CTTCTGCGCT AAGATTGTCA GTTTCCAAAA ACGAGGAGGA TTTGATATTC 1081 ACCTGGCCCG ATCTGGCCAT ACACTTGAGT GACAATGACA TCCACTTTGC CTTTCTCTCC 1141 ACAGGTGTCC ACTCCCAGGT CCAAGTTTAA ACGGATCTCT AGCGAATTCC CTCTAGAGGG 1201 CCCGTTTCTG CTAGCAAGCT TGCTAGCGGC CGCTCGAGGC CGGCAAGGCC GGATCCCCCG 1261 ACCTCGACCT CTGGCTAATA AAGGAAATTT ATTTTCATTG CAATAGTGTG TTGGAATTTT 1321 TTGTGTCTCT CACTCGGAAG GACATATGGG AGGGCAAATC ATTTGGTCGA GATCCCTCGG 1381 AGATCTCTAG CTAGAGGATC GATCCCCGCC CCGGACGAAC TAAACCTGAC TACGACATCT 1441 CTGCCCCTTC TTCGCGGGGC AGTGCATGTA ATCCCTTCAG TTGGTTGGTA CAACTTGCCA 1501 ACTGAACCCT AAACGGGTAG CATATGCTTC CCGGGTAGTA GTATATACTA TCCAGACTAA 1561 CCCTAATTCA ATAGCATATG TTACCCAACG GGAAGCATAT GCTATCGAAT TAGGGTTAGT 1621 AAAAGGGTCC TAAGGAACAG CGATGTAGGT GGGCGGGCCA AGATAGGGGC GCGATTGCTG 1681 CGATCTGGAG GACAAATTAC ACACACTTGC GCCTGAGCGC CAAGCACAGG GTTGTTGGTC 1741 CTCATATTCA CGAGGTCGCT GAGAGCACGG TGGGCTAATG TTGCCATGGG TAGCATATAC 1801 TACCCAAATA TCTGGATA
GC ATATGCTATC CTAATCTATA TCTGGGTAGC ATAGGCTATC 1861 CTAATCTATA TCTGGGTAGC ATATGCTATC CTAATCTATA TCTGGGTAGT ATATGCTATC 1921 CTAATTTATA TCTGGGTAGC ATAGGCTATC CTAATCTATA TCTGGGTAGC ATATGCTATC 1981 CTAATCTATA TCTGGGTAGT ATATGCTATC CTAATCTGTA TCCGGGTAGC ATATGCTATC 2041 CTAATAGAGA TTAGGGTAGT ATATGCTATC CTAATTTATA TCTGGGTAGC ATATACTACC 2101 CAAATATCTG GATAGCATAT GCTATCCTAA TCTATATCTG GGTAGCATAT GCTATCCTAA 2161 TCTATATCTG GGTAGCATAG GCTATCCTAA TCTATATCTG GGTAGCATAT GCTATCCTAA 2221 TCTATATCTG GGTAGTATAT GCTATCCTAA TTTATATCTG GGTAGCATAG GCTATCCTAA 2281 TCTATATCTG GGTAGCATAT GCTATCCTAA TCTATATCTG GGTAGTATAT GCTATCCTAA 2341 TCTGTATCCG GGTAGCATAT GCTATCCTCA TGATAAGCTG TCAAACATGA GAATTAATTC 2401 TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT 2461 GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT 2521 ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT 2581 TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC 2641 CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA 2701 AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG 2761 TAAGATCCTT GAGAGTT
TTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT 2821 TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG 2881 CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC 2941 GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC 3001 GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA 3061 CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC 3121 AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT 3181 AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA 3241 TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA 3301 ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA
3361 GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA
3421 TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT
3481 TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT
3541 GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG
3601 AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT
3661 AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA
3721 AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC
3781 TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC
3841 ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT
3901 TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG
3961 GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA
4021 GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT
4081 AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA
4141 TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC
4201 GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC
4261 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA
4321 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG
4381 CGAGTCAGTG AGCGAGGAAG C
//
其他哺乳动物表达载体：
pCHO1.0 pBApo-CMV-Pur pOPRSVI
pcDNA3.1/His C pcDNA5/FRT/V5-His-TOPO pREP4
pcDNA3.1/His B pcDNA5/FRT/TO-TOPO pDual-GC
pcDNA3.1/His A pcDNA5/TO pBK-RSV
pIRESpuro3 pcDNA5/FRT/TO pBK-CMV
pIRES2-EGFP pcDNA5/FRT pBI-CMV4
pTT5 pFLAG-CMV2 pcDNA4/TO/Myc-His/LacZ pNFkB-DD-tdTomato pcDNA3.1/CT-GFP-TOPO pOPI3CAT
pBI-CMV5 pcDNA3.1/NT-GFP-TOPO pGene/V5-His B pSEAP2-Basic pOptiVEC-TOPO pSwitch
pSEAP2-Control pCMV-MEKK1 pCMVLacI
pBI-CMV3 pCMV-MEK1 pVgRxR
pBI-CMV2 pCMV-PKA pIND
pBI-CMV1 pcDNA6.2/nTC-Tag-DEST pTRE3G-Luc
pNFκB-MetLuc2-Reporter pcDNA6.2/cTC-Tag-DEST pTRE3G
pCRE-MetLuc2-Reporter pcDNA3.2/V5/GW/D-TOPO pTRE2-hygro
pAcGFP1-Actin pcDNA6.2/V5/GW/D-TOPO pTRE-Tight
pAcGFP1-N In-Fusion Ready pcDNA6.2/nGeneBLAzer-GW/D-TOPO pTK-hyg
pAcGFP1-C3 pcDNA6.2/C-YFP-DEST pTet-On
pAcGFP1-C pcDNA6.2/cGeneBLAzer-DEST pTet-Off
pAcGFP1-p53 pcDNA6/V5-His A pTet on advanced pAcGFP1-Mito pcDNA6/V5-His B pRevTRE
pAcGFP1-Mem pcDNA6/V5-His C pRevTet-On
pAcGFP1-Lam pcDNA6/myc-His C pRevTet-Off pAcGFP1-Golgi pcDNA6/myc-His A pCMV-Tet3G pAcGFP1-F pcDNA6/myc-His B pTRE2
pAcGFP1-Hyg-C1 pcDNA6.2/nGeneBLAzer-DEST pBD-NF-κB pAsRed2-N1 pcDNA4/HisMax-TOPO pCMV-AD ptdTomato-N1 pcDNA6.2/nLumio-DEST pCMV-BD
pCMV-tdTomato pcDNA6.2/cLumio-DEST pBIND-Id Control pCRE-DD-tdTomato pcDNA4/myc-His C pBIND
pCMV-DsRed-Express2 pcDNA4/HisMax C pG5 luciferase
pEF1α-tdTomato pcDNA4/HisMax A pACT-MyoD
pCRE-hrGFP c-Flag pcDNA3 pACT
ptdTomato-C1 pcDNA4/HisMax B pCMV-SPORT6 pAsRed2-C1 pcDNA4/myc-His B pGL4.13
pGL3-Promoter pcDNA4/myc-His A pGL4.19
pGL3 basic pcDNA4/His C pGL4.26
pAcGFP1-C2 pcDNA4/His B pGL4.20
pAcGFP1-C1 pcDNA4/His A pGL4.29
pAcGFP1-N3 pcDNA6/TR pGL4.30
pAcGFP1-N2 pcDNA4/TO/Myc-His A pGL4.27
pAcGFP1-N1 pcDNA4/TO pGL4.75
pAcGFP1-C In-Fusion Ready pcDNA4/TO/Myc-His B pGL4.10
pCRE-DD-AmCyan1 pcDNA4/TO/Myc-His C pGRN145
pNFkB-DD-AmCyan1 pcDNA3.3-TOPO pSecTag2 A pDsRed2-Bid pBudCE4.1 pEBVHis B pDsRED2-Mito pFLAG-CMV-4 pEBVHis A
pDD-AmCyan1 Reporter pFLAG-CMV-3 pCMV-Tag 3C pAmCyan1-N1 pFLAG-CMV-2 pCMV-Tag 3A pAmCyan1-C1 pFLAG-CMV-5a pCMV-Tag 3B
pEF1α-IRES-DsRed-Express2 p3XFLAG-CMV-9 pCMV-Tag 5C
pEF1α-DsRed-Monomer-N1 p3xFLAG-CMV-10 pCMV-Tag 5A pDsRED-Monomer-N1 p3XFLAG-CMV-8 pCMV-Tag 4A pDsRed-Express-N1 p3XFLAG-CMV-7.1 pCMV-Tag 5B
p3XFLAG-CMV-7 pDsRed-Monomer-N In-Fusion Ready pCMV-Tag 4B pDsRed-Express-C1 p3XFLAG-CMV-13 pCMV-Tag 2C pIRES2-ZsGreen1 p3XFLAG-CMV-14 pCMV-Tag 2B pDsRed-Express2-C1 plRES2-ZsGreen1 pCMV-Tag 2A pDsRed-Express2-N1 pBApo-EF1α-pur pCMV-LacZ
pEF1α-DsRed-Express2 pBApo-EF1α-neo pCMV-Myc
pIRES2-DsRed-Express pBApo-CMV pEF1α-IRES-AcGFP1 pIRES2-DsRed-Express2 pBApo-CMV-neo pEF1α-IRES-ZsGreen1 pIRES-hrGFP-1a pIRES-EGFP pEF1α-AcGFP1-N1 pIRESneo2 pIRESneo3 pIRES2-DsRed2 pIRESneo pDsRed-Monomer pIRES2-AcGFP1 pIREShyg3 pIRES
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