靠背轮
Tag/PTM
Hydro-phobicity 疏水性
Net Charge 净电荷
Size/globular volume
10.8.0.254
特异性Gel filtration
Size
HIC
Hydrophobicity
Ion exchange Charge
Affinity Biorecognition
Reversed phase Hydrophobicity
Base matrix
Mechanical stability 机械稳定性
Efficiency/Resolution 效率
Mechanical properties and chemical stability
are key issues to be considered
SOURCE™15 AGAROSE Medium porosity 50-70 % 中等孔度Sintered microbead structure 烧结的微珠结构
High porosity 90-96 % 高孔度90-96%
Open structure with connected rods 纤维连接成的开放结构
High connectivity 高连结性
•Stable to
•Low non-specific adsorption
•Elastic
The Ligand The type of ligand determines the mode of chromatography. There is an optimal window for the 配基的类型决定谱的模式。有根据应用不同优化配基密度的优化窗口。
温调节纸浆模具Ion exchange (Charge)
怎么自制纳米胶带电荷
Affinity (Biorecognition)
生物识别
Reversed phase (Hydrophobicity)
疏水相互作用
HIC
(Hydrophobicity)
疏水相互作用
O Capto 系列层析介质的基架
集束线高流速琼脂糖High flow agarose •亲水性Æ蛋白和基架间的非特异吸附非常小“protein friendly”•化学稳定性•开放的孔结构
•公认的生物分离介质现在增加两大特点：
刚性更强、传质速度更快
Capto™ Q and Sepharose™ 6 Fast Flow in BPG™300, 20 cm bed height
Pressure/flow specification: >700 cm/h in 1 m diameter
column, 20 cm bed height, below 3 bar
高机械强度意味着
•在更短的时间处理更大体积的物料•每个循环的周期缩短
•目标产品的均一度更好
(DEAE)
-OCH 2CH 2N +
H(CH Quaternary aminoethyl (QAE)-OCH CH 2N +(C 2H 5(CH 3)3
Sulphopropyl Methylsulphonate weak ion exchangers:capacity varies with pH
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