HIV/AIDS患者外周血中4种细胞因子IL-2、IL-4、IL-15和IL-21表达水平及其临床意义研究作者：刘琼华 刘志生 郑煜煌 陈霞 贺波 郑力文来源：《湖南中医药大学学报》2019年第08期 〔摘要〕 目的 观察外周血 4 种细胞因子（IL-2、IL-4、IL-15及IL-21）在HIV感染者/AIDS患者（HIV/AIDS）和正常人表達水平的区别及其与HIV RNA双电源自动切换装置病毒载量和CD3+、CD4+细胞数量的相关性，分析其与HIV感染的关系和临床意义。方法 40例未经抗病毒的HIV/AIDS 和31名正常人，采用ELISA法检测外周血4种细胞因子（IL-2、IL-4、IL-15及IL-21箱包提手
），使用流式细胞计数法检测外周血CD3+、CD4+ T 淋巴细胞数量，实时聚合酶链反应测定血清中HIV RNA水平。结果 未经抗病毒的HIV/AIDS外周血IL-2、IL-15及IL-21水平低于正常人，而IL-4水平高于正常人（P<0.05）。CD3+、CD4+细胞数量和各细胞因子水平显示出一定的相关关系，IL-2和IL-15水平均与HIV-1 RNA载量呈负相关。结论 立体绣花
提示外周血4种细胞因子都可能参与了HIV感染的致病机制，并与小型塑料封口机
HIV RNA病毒载量及CD3+、CD4+细胞数量相关。 〔关键词〕 人免疫缺陷病毒;艾滋病;IL-2;IL-4;IL-15;IL-21
〔中图分类号〕R512.91 ; ; ; 〔文献标志码〕B ; ; ; 〔文章编号〕doi：10.3969/j.issn.1674-070X.2019.08.018
〔Abstract〕 Objective To observe the differences of 4 cytokine levels between HIV/
AIDS patients and normal persons， and their relevance with HIV RNA level and CD3+， CD4+ cell count， and to analyze the relationship with HIV infection and clinical significance. Methods A total of 40 HIV/AIDS patients without antiviral therapy and 31 normal persons were enrolled. Enzyme Linked Immunosorbent Assay （ELISA） was used to detect 4 cytokine levels （IL-2， IL-4， IL-15 and IL-21）. At the same time超高压电容， the number of CD3+ and CD4+ T lymphocytes in peripheral blood was detected by flow cytometry， and the level of HIV RNA in serum was determined by real-time polymerase chain reaction. Results IL-2，t型槽螺母 IL-15 and IL-21 in peripheral blood of HIV/AIDS patients without antiviral therapy were significantly lower than that of the normal people， and IL-4 level was higher than the normal people（P<0.05）. The number of CD3+， CD4+ cells and the level of each cytokine showed a certain correlation， and IL-2 and IL-15 levels were negatively correlated with HIV-1 RNA load. Conclusion It is suggested that all 4 cytokines in peripheral blood may be involved in the pathogenesis of HIV infection and are related to HIV RNA viral load and the number of CD3+ and CD4+ cells.
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