6% | 8% | 10% | 12% | 15% | ||||||
5mL | sds聚丙烯酰胺凝胶电泳10mL | 5mL | 10mL | 5mL | 10mL | 5mL | 10mL | 5mL | 10mL | |
H2O | 2.6mL | 5.3mL | 2.3mL | 4.6mL | 1.9mL | 4mL | 1.6mL | 3.3mL | 1.1mL | 2.4mL |
30% mix | 1mL | 2mL | 1.3mL | 2.7mL | 1.7mL | 3.3mL | 2mL | 4mL | 2.5mL | 5mL |
1.5M Tris pH 8.8 | 1.3mL | 2.5mL | 1.3mL | 2.5mL | 1.3mL | 2.5mL | 1.3mL | 2.5mL | 1.3mL | 2.5mL |
10% SDS | 50uL | 100uL | 50uL | 100uL | 50uL | 100uL | 50uL | 100uL | 50uL | 100uL |
10% APS | 50uL | 100uL | 50uL | 100uL | 50uL | 100uL | 50uL | 100uL | 50uL | 100uL |
TEMED | 4uL | 8uL | 3uL | 6uL | 2uL | 4uL | 2uL | 4uL | 2uL | 4uL |
Stacking Gel | 5% | |
2mL | 5mL | |
H2O | 1.46mL | 3.6mL |
30% mix | 330uL | 830uL |
1.5M Tris pH6.8 | 170uL | 420uL |
10% SDS | 20uL | 50uL |
10% AP | 20uL | 50uL |
TEMED | 2uL | 5uL |
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