plRES2-ZsGreen1
北京华越洋生物VECT6130 plRES2-‐ZsGreen1
plRES2-‐ZsGreen1载体基本信息
载体名称: plRES2-ZsGreen1
质粒类型: 哺乳动物表达载体；双顺反子载体；荧光报告载体高拷贝/低拷贝: 高拷贝 启动子: CMV
载体大小: 5.3kb
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: 无
载体抗性: 卡那霉素
筛选标记: --pgl3
备注: ZsGreen1比EGFP亮度更高；IRES元件（内部核糖体结合位点） 稳定性: --
组成型/诱导型: 组成型
病毒/非病毒: 非病毒
plRES2-‐ZsGreen1载体质粒图谱和多克隆位点信息
plRES2-‐ZsGreen1载体简介
IRES-containing bicistronic vectors allow the simultaneous expression of two proteins separately but from the same RNA transcript (1, 2).
How Does an IRES Work?
The IRES of the encephalomyocarditis virus (ECMV) permits the translation of two open reading frames from one messenger RNA. Although translation initiation of eukaryotic mRNAs occurs almost exclusively at the 5' cap, the IRES allows ribosomes to bind and initiate translation at a second, internal location. Thus, two proteins are
expressed simultaneously from the same bicistronic mRNA transcript.Clontech's pIRES Vector contains two multiple cloning sites flanking the central IRES for you to clone and express two genes of interest.pIRES Vectors Containing Antibiotic Selection Markers
The pIRES Selection Vectors pIRESpuro3, pIREShyg3, pIRESneo3, and pIRESbleo3 each contain a multiple cloning site located upstream of the IRES from which a selection marker is expressed. Because your gene of interest and a selection marker are translated from a single RNA, you can be
certain that nearly 100% of the colonies that are resistant to puromycin, hygromycin, G418, and bleomycin also express your protein of interest.
All IRES Selection Vectors contain a partially disabled IRES which reduces the efficiency of translation initiation for the selection marker relative to that of the cloned gene, and allows preferential selection of cells expressing high levels of your protein of interest (3).
Bicistronic (IRES) Fluorescent Protein Expression Vectors
Similarly, fluorescent protein-containing IRES vectors allow coexpression of your target protein and a fluorescent protein.
Successfully transfected target cells are easily identified by fluorescence microscopy or flow cytometry. The are several options for different fluorescent proteins, which include mCherry, ZsGreen1, and tdTomato (pLVX-IRES Vectors) and AcGFP1, DsRed2, and DsRed-Express (pIRES2 Vectors).
Features
Translate your gene of interest and an antibiotic resistance marker or fluorescent protein from a singl
e mRNA
Quickly identify cells expressing your protein of interest at high levels
Bicistronic expression allows faster, better stable clone selection
Choice of vectors enables screening via antibiotic selection, fluorescence microscopy, or flow cytometry
Applications
Rapidly select stable clones that show high-level expression of your target protein Identify transfected cells by antibiotic selection, fluorescence microscopy, or flow cytometry
References
Jackson, R. J. et al. (1990) Trends Biochem. Sci. 15:477–483.
Jang, S. K. et al. (1988) J. Virol. 62:2636–2643.
Rees, S. et al. (1996) BioTechniques 20:102–110.
plRES2-‐ZsGreen1其他哺乳动物表达载体：
pCHO1.0 pBApo-CMV-Pur pOPRSVI
pcDNA3.1/His C pcDNA5/FRT/V5-His-TOPO pREP4
pcDNA3.1/His B pcDNA5/FRT/TO-TOPO pDual-GC
pcDNA3.1/His A pcDNA5/TO pBK-RSV
pIRESpuro3 pcDNA5/FRT/TO pBK-CMV
pIRES2-EGFP pcDNA5/FRT pBI-CMV4
pTT5 pFLAG-CMV2 pcDNA4/TO/Myc-His/LacZ pNFkB-DD-tdTomato pcDNA3.1/CT-GFP-TOPO pOPI3CAT
pBI-CMV5 pcDNA3.1/NT-GFP-TOPO pGene/V5-His B pSEAP2-Basic pOptiVEC-TOPO pSwitch
pSEAP2-Control pCMV-MEKK1 pCMVLacI
pBI-CMV3 pCMV-MEK1 pVgRxR
pBI-CMV2 pCMV-PKA pIND
pBI-CMV1 pcDNA6.2/nTC-Tag-DEST pTRE3G-Luc
pNFκB-MetLuc2-Reporter pcDNA6.2/cTC-Tag-DEST pTRE3G
pCRE-MetLuc2-Reporter pcDNA3.2/V5/GW/D-TOPO pTRE2-hygro
pAcGFP1-Actin pcDNA6.2/V5/GW/D-TOPO pTRE-Tight
pAcGFP1-N In-Fusion Ready pcDNA6.2/nGeneBLAzer-GW/D-TOPO pTK-hyg
pAcGFP1-C3 pcDNA6.2/C-YFP-DEST pTet-On
pAcGFP1-C pcDNA6.2/cGeneBLAzer-DEST pTet-Off
pAcGFP1-p53 pcDNA6/V5-His A pTet on advanced pAcGFP1-Mito pcDNA6/V5-His B pRevTRE
pAcGFP1-Mem pcDNA6/V5-His C pRevTet-On
pAcGFP1-Lam pcDNA6/myc-His C pRevTet-Off
pAcGFP1-Golgi pcDNA6/myc-His A pCMV-Tet3G
pAcGFP1-F pcDNA6/myc-His B pTRE2
pAcGFP1-Hyg-C1 pcDNA6.2/nGeneBLAzer-DEST pBD-NF-κB
pAsRed2-N1 pcDNA4/HisMax-TOPO pCMV-AD
ptdTomato-N1 pcDNA6.2/nLumio-DEST pCMV-BD
pCMV-tdTomato pcDNA6.2/cLumio-DEST pBIND-Id Control
pCRE-DD-tdTomato pcDNA4/myc-His C pBIND
pCMV-DsRed-Express2 pcDNA4/HisMax C pG5 luciferase
pEF1α-tdTomato pcDNA4/HisMax A pACT-MyoD
pCRE-hrGFP c-Flag pcDNA3 pACT
ptdTomato-C1 pcDNA4/HisMax B pCMV-SPORT6 pAsRed2-C1 pcDNA4/myc-His B pGL4.13
pGL3-Promoter pcDNA4/myc-His A pGL4.19
pGL3 basic pcDNA4/His C pGL4.26
pAcGFP1-C2 pcDNA4/His B pGL4.20
pAcGFP1-C1 pcDNA4/His A pGL4.29
pAcGFP1-N3 pcDNA6/TR pGL4.30
pAcGFP1-N2 pcDNA4/TO/Myc-His A pGL4.27
pAcGFP1-N1 pcDNA4/TO pGL4.75
pAcGFP1-C In-Fusion Ready pcDNA4/TO/Myc-His B pGL4.10
pCRE-DD-AmCyan1 pcDNA4/TO/Myc-His C pGRN145
pNFkB-DD-AmCyan1 pcDNA3.3-TOPO pSecTag2 A pDsRed2-Bid pBudCE4.1 pEBVHis B pDsRED2-Mito pFLAG-CMV-4 pEBVHis A
pDD-AmCyan1 Reporter pFLAG-CMV-3 pCMV-Tag 3C pAmCyan1-N1 pFLAG-CMV-2 pCMV-Tag 3A pAmCyan1-C1 pFLAG-CMV-5a pCMV-Tag 3B
pEF1α-IRES-DsRed-Express2 p3XFLAG-CMV-9 pCMV-Tag 5C
pEF1α-DsRed-Monomer-N1 p3xFLAG-CMV-10 pCMV-Tag 5A pDsRED-Monomer-N1 p3XFLAG-CMV-8 pCMV-Tag 4A pDsRed-Express-N1 p3XFLAG-CMV-7.1 pCMV-Tag 5B
p3XFLAG-CMV-7 pDsRed-Monomer-N In-Fusion Ready pCMV-Tag 4B pDsRed-Express-C1 p3XFLAG-CMV-13 pCMV-Tag 2C pIRES2-ZsGreen1 p3XFLAG-CMV-14 pCMV-Tag 2B pDsRed-Express2-C1 plRES2-ZsGreen1 pCMV-Tag 2A pDsRed-Express2-N1 pBApo-EF1α-pur pCMV-LacZ
pEF1α-DsRed-Express2 pBApo-EF1α-neo pCMV-Myc
pIRES2-DsRed-Express pBApo-CMV pEF1α-IRES-AcGFP1 pIRES2-DsRed-Express2 pBApo-CMV-neo pEF1α-IRES-ZsGreen1 pIRES-hrGFP-1a pIRES-EGFP pEF1α-AcGFP1-N1 pIRESneo2 pIRESneo3 pIRES2-DsRed2 pIRESneo pDsRed-Monomer pIRES2-AcGFP1 pIREShyg3 pIRES
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