DATA SHEET
Reagent: TZM-bl
钢锭模Catalog Number:8129
Lot Number:130106
Release Category:E
Provided: 1 ml/vial (4.0 x 106 cells/vial). Viability is 93%.
防砸安全鞋Cell Type:TZM-bl, previously designated JC53-bl (clone 13) is a HeLa cell line. The parental cell
line (JC.53) stably expresses large amounts of CD4 and CCR5. The TZM-bl cell line was投料机
generated from JC.53 cells by introducing separate integrated copies of the luciferase
and ß-galactosidase genes under control of the HIV-1 promoter. The TZM-bl cell line is
highly sensitive to infection with diverse isolates of HIV-1.
PLEASE NOTE: This TZM-bl cell line is contaminated with ecotropic murine
配置管理系统leukemia virus, MLV. For additional information please consult the references below.
Propagation
Medium:
DMEM (90%), 10% FBS, 100 units of Penicillin and 0.1 mg/ml of Streptomycin.
Freeze Medium:50% FBS, 40% DMEM, 10% DMSO.建筑
线脚 Growth Characteristics:Adherent cell line. No special requirements for thawing and reestablishing th
e culture. Cells grow in single cell layer. Antibiotic selection is not required to maintain stable expression of the receptors and reporter genes.
Sterility:Negative for bacteria, mycoplasma, and fungi.
Special Characteristics:The TZM-bl indicator cell line enables simple and quantitative analysis of HIV using either β-gal or luciferase as a reporter. It is maximally sensitive to HIV infection by including DEAE-dextran in the infection medium. The ß-gal and luciferase genes are also
induced by HIV-2 infection.
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