常用蛋白酶切割位点

常用融合蛋白切割位点

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PROTEASE	COMPANY	Protease Capture
Thrombin Leu-Val-Pro-Arg▼Gly-Ser L-V-P-R▼G-S	Amersham-Biosciences, Novagen, SIGMA, Roche	肉食加工Benzamidine-Agarose
Factor Xa Ile-Glu/Asp-Gly-Arg▼? I-E/D-G-R▼	Amersham-Biosciences, New England Biolabs, Roche	Benzamidine-Agarose
Enterokinase Asp-Asp-Asp-Asp-Lys▼ D-D-D-D-K▼	New England Biolabs, Novagen, Roche	Trypsin Inhibitor-Agarose
TEV protease Glu-Asn-Leu-Tyr-Phe-Gln▼Gly E-N-L-Y电压跟随器电路图–F-Q▼G	Invitrogen – Life Technologies	Ni-NTA (6His recomb. TEV)
PreScission Leu-Glu-Val-Leu-Phe-Gln▼Gly-Pro L-E-V-L-F-Q▼G-P	Amersham-Biosciences	GSTrap for GST fusion enzyme
TAGZyme His-tag removal by Exoproteolytic Digestion 主轴加工	Qiagen	Ni-NTA (6His recomb. enzyme)
Intein Site dithiothreitol cleavage	New England Biolabs	DTT elimination by dialysis?
HRV 3C Protease Leu-Glu-Val-Leu-Phe-Gln▼Gly-Pro L-E-V-L-F-Q▼G-P	Novagen	Ni-NTA (6His recomb. enzyme)
SUMO Protease recognize the tertiary structure of the ubiquitin-like (UBL) protein, SUMO	Invitrogen – Life Technologies, LifeSensors	Ni-NTA (6His recomb. enzyme)
Kex-2 -Arg-X-Lys/Arg-Arg▼	Invitrogen – Life Technologies,	Ni-NTA (6His recomb. enzyme) KEX2对arg的专一性高，要求最重要。 Arg前为lys效率最高，不切-Arg-lys，Pro影响KEX2切割
羧肽酶		羧肽酶B可以切割C端的Lys或Arg；羧肽酶A可以切割C端除了Lys、Arg、Pro的氨基酸，但如果倒数第二个氨基酸为Pro两种羧肽酶均不能作用