• 694 •http：//www. chinagp. net E-mail：zgqkyx@ chinagp. net, cn c(SIP C hinese General Practice
February 2017, Vol,20 No.6
论著
张海燕\张淼\吕顺武2，高亮\李建智\谢世平^
【摘要】目的明确艾滋病湿热内蕴证蛋白水平的表达差异，探索凝血与补体级联反应信号通路在中医证候实
质中所主导的作用。方法2014年8月根据整随机抽样原则，纳入艾滋病患者48例（艾滋病肺脾气虚证25例为艾
滋病肺脾气虚证组，艾滋病湿热内蕴证23例为艾滋病湿热内蕴证组），并以同地区人口资料相似的非HIV感染者16例
骑行者
为对照组，采集所有受试者血清进行iTRAQ技术研究，将差异蛋白进行通路注释。结果艾滋病湿热内蕴证组与艾滋
碳浆病肺脾气虚证组相比，a l-抗胰蛋白酶、补体C4结合蛋白（C4bP)表达下调；艾滋病湿热内蕴证组与对照组相比，
a l-抗胰蛋白酶、补体C4bp、a-2巨球蛋白、人结合丝氨酸凝集素蛋白酶2表达上调，补体Clr subcomponent、补体
因子B及载脂蛋白B variant表达下调。这些差异蛋白均存在于凝血与补体级联反应信号通路。结论艾滋病湿热内蕴
证的发病机制可能是通过凝血与补体级联反应信号通路实现的，该信号通路上的差异蛋白点可作为艾滋病临床的
潜在用药靶点。
【关键词】获得性免疫缺陷综合征；湿热；蛋白质组学
【中图分类号】R 512. 91 【文献标识码】 A DOI：10. 3969/j. i n. 1007 -9572.2017.06.012
张海燕，张淼，吕顺武，等.凝血与补体级联反应信号通路对艾滋病湿热内蕴证发病的影响[J].中国全科医
阿伐那非的作用与功效学，2017, 20 (6)：694 -698. [www.chinagp]
ZHANG H Y, ZHANG M, LYU S W, et al. Impact of signal path of coagulation and complement cascades reaction for
AIDS patients with syndrome of interior dampness - heat [ J].Chinese General Practice, 2017, 20 (6)：694 -698.
Impact of Signal Patli of Coagulation and Complement Cascades Reaction for AIDS Patients witli Syndrome of Dampness - heat ZHANG Hai - yan1 , ZHANG Miao1 , LYU Shun-w u , GAO Liang1 , L ljia n-z h i1, XIE Shi -p ing1 *
1. Henan University of Traditional Chinese Medicine , Zhengzhou 450046 , China
2. Luoyanp No.1 Hospital of TCM , Luoyanp 47 1000 , China
* Correspondinp author：XIE Shi - pinp , Professor; E-m a il ：xspzz@ 126. corm
【Abstract】Objective To make clear the expression differences of the protein levels in the syndrome of interior
dampness - heat of AIDS , and explore the role of signal path of coagulaton and complement TCM syndrome. Methods By random cluster sampling principle , 48 AIDS patients (25 with syndrome of Qi deficiency of lung
and spleen of AIDS was Qi deficiency of lung and spleen of AIDS group , 23 with syndrome of interior dampness - heat of AIDS
was interior dampness - heat of AIDS group) were enrolled in October 2014 , and non HIV infected individuals with similar
demographic data in the same area were taken as the control group. The serum samples of all the subjects were studied with iTRAQ
techniques , and the differential proteins were annotated by signal pathway. Results Compared with Qi deficiency of lung and
spleen of AIDS group , the expression of a1 - antitrypsin and complement C4bp was down - regulate
d in interior dampness - heat
of AIDS group. Compared with the control group , the expression of a1 - antitrypsin , complement C4bp , a - 2 macroglobulin and
human - binding serine lectin protease 2 was up - regulated and the expression of C1r subcomponent complement , complement手势控制
factor B , apolipoprotein B variant was down - regulated in interior dampness - heat of AIDS group. These proteins activated in
coagulation and complement cascades reaction signal path. Conclusion The pathogenesis of AIDS patients suffering from
基金项目：国家十二五重大专项—HIV/AIDS中医证候转录组学研究（2012ZX10005001 -005)；河南省高等学校重点科研项目
—基于差异基因建立HIV/AIDS湿热内蕴证判别模型（15A360038)；河南中医学院博士科研基金—艾滋病中医发病机制“艾毒
飞行棋棋盘伤元”假说的物质基础的文献及实验研究（BSJJ2012 -24)
1. 450046河南省郑州市，河南中医药大学
2. 471000河南省洛阳市第一中医院网约车管理系统
*通信作者：谢世平，教授；E - mail ：xspzz@ 126. com
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