才直紿碌妁2019,45(4):54-60Plant Protection
中山一中协同办公国产Bt-CrylAb和Bt-(CrylAb+VipAa)玉米对
张丹丹，吴孔明$
支承板
(中国农业科学院植物保护研究所，植物病虫害生物学国家重点实验室，北京100193)摘要草地贪夜蛾Spodoptera frugiperda(J. E.Smith)是世界性重大农业害虫。种植转基因Bt作物是主要的防治手段之一。我们利用室內生物测定方法评价了国产Bt-Cry1Ab玉米(转化体C0030.3.5)和Bt-(Cry1Ab+ Vip3Aa)玉米(转化体DBN3601和DBN3608)对草地贪夜蛾1〜4龄幼虫的毒力。结果显示，供试两种转基因玉米共6个品种皆可高效表达目标杀虫蛋白并对草地贪夜蛾具有很强的毒杀作用，对1龄幼虫的致死率达到59%〜100%，存活幼虫的生长发育亦受到显著抑制。表明国內研发的Bt-Cry1Ab玉米和Bt-(Cry1Ab+Vip3Aa)玉米对草地贪夜蛾具有良好的控制效果，其中Bt-(Cry1A\gVip3Aa)玉米的防治效果显著优于Bt-Cry1Ab玉米，两种转基因玉米皆具有较好的商业化应用前景。关键词草地贪夜蛾；Bt-Cry1Ab玉米；Bt-(Cry1Ab+Vi P3Aa)玉米；控制效果
中图分类号：S435.132文献标识码：A DOI：10.16688/j.zwbh.2019251e通话
钨铜电触头
The bioassay of Chinese domestic Bt-CrylAb and Bt-(CrylAb+Vip3Aa) maize against the fall army w orm,Spodoptera frugiperda
dota重金属ZHANG Dandan,WU Kongming
(.State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant
Protection,Chinese Academy of Agricultural Sciences,Beijing100193,China)
Abstract The fall armyworm,Spodoptera frugiperda(J.E.Smith)is an important agricultural pest in the world and planting Bt crop has been widely deployed for the pest control in its native region,the American continent.In this study,the control efficacy of Chinese domestic Bt-CrylAb maize(Event-C0030.3.5)and Bt-(CrylAb+ Vip3Aa)maize(Event-DBN3601and DBN3608)to1st-4th instar larvae of fall armyworm were evaluated by the method of feeding on maize leaves in the lab.The results showed that all the six varieties of two kinds Bt corns could express the target insecticidal proteins efficiently and have strong toxicity to the pest.The insecticidal eff--cacy to the1st instar larvae reached59%—100%,and the growth and development of the survived larvae were a--so significantly inhibited.Our results indicated that Bt-CrylAb and Bt-(CrylAb+Vip3Aa)maize developed in Ch0nahadh0ghcontroleff0cacyaga0nstfa l armyworm.However,compared w th the s0ngle Bt gene m
篮球打气筒a0ze,the
controleff0cacyofthetwoBtgenema0zewassgn0fcantlyh0gher.ItsconcludedthatthetwoBtma0zepresent goodcommerc0alapplcatonprospects.
Key words Spodoptera frugiperda+Bt-CrylAb maize；B--(CrylAb+Vip3Aa)maize；control efficacy
草地贪夜蛾Spodoptera frugiperda(J. E. Smith)是一种原分布于美洲大陆热带和亚热带地区的杂食性害虫口*，其寄主植物多达76个科，353种囚。同其他重大迁飞性农业害虫一样，具有“国际性、迁飞性、暴发性和毁灭性”的特点3，目前已成为世界范围内严重威胁粮食安全的重大生物灾害。其幼虫取食为害玉米，低龄幼虫取食玉米叶片可造成“窗孔”形症状。高龄幼虫除了取食叶片造成孔洞外，还为害心叶、叶鞘、雄穗和果穗等部位4，对玉米产量造成严重影响。据报道,20世纪60年代，在阿
收稿日期：2019-05-17修订日期：2019-05-22
基金项目：转基因生物新品种培育科技重大专项(2016ZX08012-004)通信作者E-mail:wukongming®caas
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